Dong Hwan Lee, Si Young Ha, Ji Young Jung, Jae-Kyung Yang
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영어(ENG)
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https://www.earticle.net/Article/A397107
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영어
Roridin E, produced by Podostroma cornu-damae, is a mycotoxin with anticancer activity. To increase the content of roridin E, submerged culture conditions were optimized using response surface methodology. Three factors, namely, medium initial pH, incubation time and agitation speed were optimized using a Box–Behnken design. The optimum submerged culture conditions to increase the content of roridin E included a medium with an initial pH of 4.0, an incubation time of 12.90 days, and an agitation speed of 63.03 rpm. The roridin E content in the submerged culture, under the aforementioned conditions, was 40.26 mg/L. The findings of this study can help lower the current price of roridin E and promote its related research.
목차
ABSTRACT INTRODUCTION MATERIALS AND METHODS Reagents and standards Microorganism Culture media Submerged Culture Determination of mycelial dry weight Determination of roridin E Experimental design by the RSM Statistical analysis RESULTS AND DISCUSSION Effect of various media on mycelial dry weight and roridin E production One-factor-at-a-time experiment Effect of incubation time on roridin E production Effect of initial pH on roridin E production Effect of agitation speed on roridin E production Optimization of roridin E production by the RSM ACKNOWLEDGEMENTS REFERENCES
Dong Hwan Lee [ Division of Environmental Forest Science and Institute of Agriculture & Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea ]
Si Young Ha [ Division of Environmental Forest Science and Institute of Agriculture & Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea ]
Ji Young Jung [ Division of Environmental Forest Science and Institute of Agriculture & Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea ]
Jae-Kyung Yang [ Division of Environmental Forest Science and Institute of Agriculture & Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea ]
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