Earticle

초록

영어

Purpose: As the demand for natural cosmetics increases, properly managing production and quality control should be prioritized. Chlorogenic acid (CGA), epicatechin gallate (ECG), and rosmarinic acid (RMA) were selected to validate the cosmetic composite LOL, a mixture of 50% EtOH extract of Ligularia stenocephala (LS), Orostachys japonica (OJ), and Lavandula angustifolia (LA). Methods: Highperformance liquid chromatography with a diode array detector (HPLC-DAD) was used to simultaneously analyze the three LOL compounds from LOL (Ligularia stenocephala , Orostachys japonica , Lavandula angustifolia ). Linearity, accuracy, and precision analyses were performed using HPLC-DAD, and the anti-oxidative effects were evaluated through a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging assay. Results: All three extracts showed a high DPPH radical-scavenging effect, and the detected marker compounds showed significant linearity (R2≥0.9997). The limit of detection (LOD) of CGA, ECG, and RMA was 0.83 μg/mL, 0.33 μg/mL, and 0.20 μg/mL, respectively. Meanwhile, the limit of quantification (LOQ) of CGA, ECG, and RMA was 2.51 μg/mL, 0.99 μg/mL, and 0.61 μg/mL, respectively. Method validation showed acceptable precision (intra-and inter-day precision, CGA, ECG, and RMA, 1.77, 1.42, 0.80% and 1.09, 0.72, 1.42%, respectively) and recovery (CGA, ECG, and RMA, 102.00%–117.78%, 94.40%-108.06%, and 93.79%–107.05%). Conclusion: The contents of the three markers were 13.99 μg/mL CGA, 8.28 μg/mL ECG, and 8.40 μg/mL of RMA, respectively. This study also suggests that the developed method can validate the LOL cosmetic composition quality control process.

한국어

목적: 천연 화장품에 대한 수요가 증가함에 따라 적절한 생산 관리 및 품질 관리가 우선 되어야 한다. Chlorogenic aicd (CGA), epicatechin gallate (ECG) 및 rosmarinic acid (RMA)을 지표성분으로 선택하여 Ligularia stenocephala (LS; 곤달비), Orostachys japonica (OJ; 와송) 및 Lavandula angustifolia (LA; 라벤더) 50 % EtOH 추출물이 함유 된 화장품 조성물 LOL을 검증했다. 방법: High-performance liquid chromatography with diode array (HPLC-DAD)를 사용하여 LOL (Ligularia stenofelphala, Orostachys japonica, Lavandula anfustifolia)의 세 가지 화합물을 동시에 분석했다. HPLC-DAD를 사용하여 선형성, 정확도 및 정밀도 분석 을 수행했다. 그리고 DPPH 라디칼 소거 분석을 통해 항산화 효과를 평가했다. 결과: 세 가지 추출물 모두 DPPH 라디칼 소거 효과 가 높았으며 검출 된 지표 성분은 유의 한 직선성을 나타냈다(R2≥0.9997). CGA, ECG 및 RMA의 검출 한계(LOD)는 각각 0.83 µg/ mL, 0.33 µg/mL 및 0.20 µg/mL이며, CGA, ECG 및 RMA의 정량 한계(LOQ)는 각각 2.51 µg/mL, 0.99 µg/mL 및 0.61 µg/mL이 다. 분석법 검증 결과, CGA, ECG 및 RMA의 허용 가능한 정밀도(일중 및 일간 정밀도)는 각각 1.77, 1.42, 0.80 % 및 1.09, 0.72, 1.42 %이고, 회수율은 각각 102.00 %-117.78 %, 94.40 %-108.06 % 및 93.79 %-107.05 %이다. 결론: 3 개의 지표 성분의 함량 은 각각 13.99 µg/mL CGA, 8.28 µg/m ECG 및 8.40 µg/mL RMA 이다. 또한 본 연구는 개발 된 방법이 LOL 화장품 조성물 품질 관리 프로세스를 검증 할 수 있음을 시사한다

중국어

目的: 随着对天然化妆品需求的增加，应优先考虑妥善管理生产和质量控制。选择绿原酸(CGA)、表儿茶素没食 子酸酯(ECG)和迷迭香酸(RMA)来验证化妆品复合物 LOL，这是一种由 50% 乙醇提取物的窄头胡瓜 (LS)、粳稻 (OJ) 和狭叶薰衣草的混合物（LA）。方法: 采用带二极管阵列检测器的高效液相色谱法（HPLC-DAD）同时分析 来自 LOL（Liguaria stenocephala 、Orostachys japonica 、Lavandula angustifolia ）的三种 LOL 化合物。使 用 HPLC-DAD 进行线性、准确度和精密度分析，并通过 DPPH 自由基清除试验评估抗氧化作用。结果: 三种提 取物均显示出较高的 DPPH 自由基清除作用，且检测到的标记化合物呈显着线性（R2≥0.9997）。CGA、ECG 和 RMA 的检测限 (LOD) 分别为 0.83 μg/mL、0.33 μg/mL 和 0.20 μg/mL。同时，CGA、ECG 和 RMA 的定量 限 (LOQ) 分别为 2.51 μg/mL、0.99 μg/mL 和 0.61 μg/mL。方法验证显示，CGA、ECG 和 RMA的可接受的精 密度（日内和日间精密度)分别为 1.77、1.42、0.80% 和 1.09、0.72、1.42%; CGA、ECG 和 RMA的回收率分别 为102.00%– 117.78%、94.40%-108.06% 和 93.79%–107.05%。结论: 三种标志物的含量分别为 13.99 μg/mL (CGA)、8.28 μg/mL (ECG) 和 8.40 μg/mL (RMA)。这项研究还表明，所开发的方法可以验证 LOL 化妆品成分的 质量控制过程。

목차

Abstract
Introduction
Methods
1. Preparation of materials
2. Reagents
3. DPPH radical scavenging assay
4. Pretreatment of samples
5. Instrumentation and Chromatographic conditions
6. Methodology validation
7. Linearity and range
8. Specificity
9. Accuracy and precision
10. Limit of detection (LOD) and limit of quantification (LOQ)
11. Content evaluation
Result and Discussion
1. DPPH radical-scavenging activity
2. Qualitative and quantitative analysis of marker compounds.
3. Establishment of simultaneous analysis conditions
4. Specificity
5. Linearity, LOD and LOQ
6. Recovery
7. Accuracy and precision
Conclusion
References
국문초록
中文摘要

키워드

곤달비 와송 라벤더 HPLC-DAD 밸리데이션 Ligularia stenocephala Orostachys japonica Lavandula angustifolia HPLC-DAD Validation Ligularia stenocephala ，粳稻，狭叶薰衣草，HPLC-DAD，验证

저자

• Ju Eun Kim [ 김주은 | College of Pharmacy, Woosuk University, Wanju-gun, Jeolabuk-do, Korea, Institute of Smart Convergence Life-Care, Woosuk University, Wanju-gun, Jeolabuk- do, Korea ]
• Abinash Chandra Shrestha [ 阿比纳什 钱德拉 什雷斯塔 | College of Pharmacy, Woosuk University, Wanju-gun, Jeolabuk-do, Korea ]
• Youn Jeong Jo [ 조윤정 | College of Pharmacy, Woosuk University, Wanju-gun, Jeolabuk-do, Korea ]
• Jae Yoon Leem [ 임재윤 | College of Pharmacy, Woosuk University, Wanju-gun, Jeolabuk-do, Korea, Institute of Smart Convergence Life-Care, Woosuk University, Wanju-gun, Jeolabuk- do, Korea ] Corresponding Author

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