Earticle

초록

영어

With the recent rapid improvement in the standards of life and westernization of dietary lifestyles, the consumption of high-calorie diets such as high-fat and high-protein red meat and instant foods has increased, while less vegetables containing dietary fiber are consumed. In addition to that, stress, erroneous dietary behaviors, and contaminated environments are linked to the risk of developing ulcerative colitis, which is on the rise. Another cause of ulcerative colitis is that involve laxative abuse, including repeated, frequent use of laxatives, and include such conditions as deteriorated bowel function, irritable bowel syndrome, diarrhea, intestinal inflammation, etc. The present study aimed to investigate the comparative evaluation of pharmacological efficacy between sulfasalazine alone and combination with herbal medicine on dextran sodium sulfate (DSS)-induced UC in mice. Balb/c mice received 5% DSS in drinking water for 7 days to induce colitis. Animals were divided into five groups (n = 9): group I-normal group, group II-DSS control group, group III-DSS + sulfasalazine (30 mg/kg), group IV-DSS + sulfasalazine (60 mg/kg), group V-DSS + sulfasalazine (30 mg/kg) + Radish Extract mixture (30 mg /kg) (SRE). DSS-treated mice developed symptoms similar to those of human UC, such as severe bloody diarrhea and weight loss. SRE supplementation, as well as sulfasalazine, suppressed colonic length and mucosal inflammatory infiltration. In addition, SRE treatment significantly reduced the expression of pro-inflammatory signaling molecules through suppression both mitogen-activated protein kinases (MAPK) and nuclear factor-kappa B (NF-B) signaling pathways, and prevented the apoptosis of colon. Moreover, SRE administration significantly led to the up-regulation of antioxidant enzyme including SOD and Catalase. This is the first report that Radish extract mixture combined with sulfasalazine protects against experimental UC via the inhibition of both inflammation and apoptosis, very similar to the standard-of-care sulfasalazine.

목차

Abstract
1. Introduction
2. Experiment Materials and Methods
2.1. Preparation of Processed Extract
2.2. Preparation of Experimental Animals and Induction of Ulcerative Colitis
2.3. Measurement of Expression of Reactive Oxygen Species (SOS) and NADPH Oxidases in Serum
2.4. Evaluation of Expression of Pro-Inflammatory Cytokaines
2.5. Evaluation of Effect on Apoptosis
2.6. Statistical analysis
3. Result and Discussion
3.1. Effects of Ulcerative colitis (UC) and anti-inflammatory
3.2. Effect of supercritical heat-treated radish extract(SRE) on the Expression of ROS and NADPH Oxidase in Serum.
3.3. ROS overexpression activates MAPK including p-p38 and p-ERK and NF-κB
3.4. Pro-inflammatory
3.5. Apoptosis
5. Conclusion
References

키워드

저자

• Hyun-Kyoung Kim [ Professor, Department of Food Science and Engineering, Seowon University, Cheongju, Korea ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  International Journal of Internet, Broadcasting and Communication

• 간기

  계간

• pISSN

  2288-4920

• eISSN

  2288-4939

• 수록기간

  2009~2025

• 십진분류

  KDC 326 DDC 380