Earticle

초록

영어

Kisspeptin, a neuropeptide and the master controller of reproductive axis upstream to GnRH neurons, and its receptor are also expressed in extrahypothalamic tissues, such as ovaries. As systemic kisspeptin has been shown to modulate follicular dynamics in cattle, we hypothesized that kisspeptin has direct actions on the ovarian follicular development. We also hypothesized that kisspeptin regulation of primordial follicle development is via modulation of VEGF expression. In order to test these hypotheses, we cultured caprine ovarian cortical strips in vitro for 7 days with supplementation of kisspeptin at 1, 10 and 100 μM concentration and observed the development of primordial follicles into intermediate, primary and secondary follicles. We also studied the alteration in the expression profile of VEGF and VEGF transcript variant 2 mRNA during follicular development in the presence of kisspeptin. We confirmed the presence of GPR54 in goat ovaries in our preliminary studies. Supplementation of kisspeptin at 1 and 10 μM concentration facilitated the development of primordial follicles into intermediate, primary and secondary follicles with less number of degenerated follicles while the same at 100 μM resulted in degeneration of follicles. We observed a drastic increase in the expression profile of VEGF and VEGF transcript variant 2 mRNA upon culture which was independent of kisspeptin treatment. In conclusion, our studies show that kisspeptin facilitates ovarian primordial development in vitro.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Collection of ovarian cortical strips
In vitro culture
Histology and assessment of follicle development
Western blot
Quantitative PCR for Kiss1, GPR54, VEGF and VEGF Trans Var2 mRNA
Statistics
RESULTS
GPR54 expression in caprine ovary
Effect of kisspeptin on primordial follicle development
Kisspeptin and VEGF expression profile during primordial follicle development
DISCUSSION
CONFLICTS OF INTEREST
FUNDING
ACKNOWLEDGEMENTS
AUTHOR CONTRIBUTIONS
AUTHOR’S POSITION AND ORCID NO.
REFERENCES

키워드

저자

• Manjula Priyantha Sumith Magamage [ National Institute of Animal Biotechnology, Hyderabad 500049, India, Department of Livestock Production, Sabaragamuwa University, Belihuloya RN 70190, Sri Lanka ]
• Sriravali Sathagopam [ National Institute of Animal Biotechnology, Hyderabad 500049, India ]
• Kiran Avula [ National Institute of Animal Biotechnology, Hyderabad 500049, India ]
• Di Neththi Nimesh Madushanka [ Department of Livestock Production, Sabaragamuwa University, Belihuloya RN 70190, Sri Lanka ]
• Sathya Velmurugan [ National Institute of Animal Biotechnology, Hyderabad 500049, India ] Corresponding Author

참고문헌

발행기관

간행물

관심저널 등록

• 간행물명

  Journal of Animal Reproduction and Biotechnology

• 간기

  계간

• pISSN

  2671-4639

• eISSN

  2671-4663

• 수록기간

  2019~2026

• 십진분류

  KDC 527 DDC 636