Jae Yong Yoo, Ki Seong Ko, Bich Ngoc Vu, Seok Han Yoon, Kyun Oh Lee
언어
영어(ENG)
URL
https://www.earticle.net/Article/A390457
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원문정보
초록
영어
The most abundant N-glycan in plants is the paucimannosidic N-glycan with core β 1,2-xylose and α1,3-fucose residues (XylMan3FucGlcNAc2). Here, we investigated the physiological significance in Arabidopsis thaliana of α-1,6-mannosyl-glycoprotein 2--N-acetylglucosaminyltransferase (EC 2.4.1.143, N-acetylglucosaminyltransferase II [GnTII]), which transfers N-acetylglucosamine (GlcNAc) residue from the nucleotide sugar donor UDP-GlcNAc to the α1,6-mannose residue of the di-antennary N-glycan acceptor in the Golgi apparatus. We assessed the phenotypic effects of a loss-of-function mutation (gnt2-1) in GnTII, and used quantitative PCR (qPCR) to examine gene expression and callose deposition assays under stress conditions. Prolonged stress from tunicamycin or NaCl treatment enhanced GnTII expression in the wild type and caused severely disrupted development and abnormal callose deposition in root tissues in the gnt2-1 mutant. Lack of the 6-arm β1,2-linked GlcNAc residue in the N-glycans promoted the unfolded protein response (UPR) upon prolonged endoplasmic reticulum (ER) stress in gnt2-1. Thus, GnTII's promotion of the 6-arm GlcNAc addition to N-glycans is important for plant growth and development under stress conditions.
저자
Jae Yong Yoo [ Division of Life Science, Division of Applied Life Sciences (BK21 Program), and Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Gyeongsang National University, 501 Jinju-daero, Jinju 52828, Korea ]
Ki Seong Ko [ Division of Life Science, Division of Applied Life Sciences (BK21 Program), and Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Gyeongsang National University, 501 Jinju-daero, Jinju 52828, Korea ]
Bich Ngoc Vu [ Division of Life Science, Division of Applied Life Sciences (BK21 Program), and Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Gyeongsang National University, 501 Jinju-daero, Jinju 52828, Korea ]
Seok Han Yoon [ Division of Life Science, Division of Applied Life Sciences (BK21 Program), and Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Gyeongsang National University, 501 Jinju-daero, Jinju 52828, Korea ]
Kyun Oh Lee [ Division of Life Science, Division of Applied Life Sciences (BK21 Program), and Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Gyeongsang National University, 501 Jinju-daero, Jinju 52828, Korea ]
본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.
간행물
간행물명
한국당과학회 학술대회
간기
연간
수록기간
2006~2022
십진분류
KDC 517DDC 614
이 권호 내 다른 논문 / 한국당과학회 학술대회 2020 한국당과학회 연례학술대회(온라인)