Damee Park, Heeji Shin, Tae Hyeon Park, Jeong-Rok Song, Tae-Jip Kim
언어
영어(ENG)
URL
https://www.earticle.net/Article/A390449
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원문정보
초록
영어
endo-(1,5)-α-L-Arabinanase (AN, EC 3.2.1.99) is an endo-acting hydrolase which can randomly hydrolyze the internal α-(1,5)-L-arabinofuranosidic linkages of arabinan polymers. The arabinan-utilization gene cluster was found from the genome of Streptococcus equinus, and a probable extracellular endo-AN GH43 (SteqAN43B) gene was cloned and heterologously expressed in Escherichia coli. The corresponding open reading frame consists of 2,283 nucleotides encoding 761 amino acids. This enzyme possesses the longer N- and C-terminal domains, compared with common bacterial ANs. Although the debranched (linear) arabinan is the most preferred substrate, SteqAN43B showed the significant hydrolytic activity on sugar beet (branched) arabinan as well. Its modes of action are suitable for the production of various branched arabinooligosaccharides from sugar beet arabinan, and the synergistic hydrolysis of arabinans in combination with the other exo-AFs.
저자
Damee Park [ Brain Korea 21 Center for Bio-Health Industry, and Division of Animal, Horticultural and Food Sciences, Graduate School of Chungbuk National University, Cheongju 28644, Korea ]
Heeji Shin [ Brain Korea 21 Center for Bio-Health Industry, and Division of Animal, Horticultural and Food Sciences, Graduate School of Chungbuk National University, Cheongju 28644, Korea ]
Tae Hyeon Park [ Brain Korea 21 Center for Bio-Health Industry, and Division of Animal, Horticultural and Food Sciences, Graduate School of Chungbuk National University, Cheongju 28644, Korea ]
Jeong-Rok Song [ Brain Korea 21 Center for Bio-Health Industry, and Division of Animal, Horticultural and Food Sciences, Graduate School of Chungbuk National University, Cheongju 28644, Korea ]
Tae-Jip Kim [ Brain Korea 21 Center for Bio-Health Industry, and Division of Animal, Horticultural and Food Sciences, Graduate School of Chungbuk National University, Cheongju 28644, Korea ]
본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.
간행물
간행물명
한국당과학회 학술대회
간기
연간
수록기간
2006~2022
십진분류
KDC 517DDC 614
이 권호 내 다른 논문 / 한국당과학회 학술대회 2020 한국당과학회 연례학술대회(온라인)