Tae Hyeon Park, Chang-Yun Choi, Jeong-Rok Song, Damee Park, Tae-Jip Kim
언어
영어(ENG)
URL
https://www.earticle.net/Article/A390447
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원문정보
초록
영어
From the genome of Lactobacillus shenzhenensis, the probable genes encoding five α -L-arabinofuranosidases (AF, EC3.2.1.55) and an β-L-arabinopyranosidase (AP, EC3.2.1.88) were found and cloned in Escherichia coli. Except LbshAF-B1, five genes were functionally expressed and characterized in this study. The comparative studies on the hydrolytic modes of action revealed that these exo-hydrolases can play the important roles in the enzymatic saccharification of arabinan polymers into L-arabinose. LbshAF-A GH51 could hydrolyze both α-(1,5)- and α-(1,2)/(1,3)-linkages in arabinan. LbshAF-B2 GH51 showed only α-(1,2)/(1,3)-specific debranching activity to generate linear products, whereas LbshAF-C1 and C2 GH43 could exclusively hydrolyze α-(1,5)-linked arabinan backbone. LbshAP showed the activity against p-nitrophenyl arabinopyranoside. It was proposed that the synergistic actions of these exo-hydrolases can facilitate the complete degradation of sugar beet arabinan to L-arabinose.
저자
Tae Hyeon Park [ Brain Korea 21 Center for Bio-Health Industry, and Division of Animal, Horticultural and Food Sciences, Graduate School of Chungbuk National University, Cheongju 28644, Korea ]
Chang-Yun Choi [ Brain Korea 21 Center for Bio-Health Industry, and Division of Animal, Horticultural and Food Sciences, Graduate School of Chungbuk National University, Cheongju 28644, Korea ]
Jeong-Rok Song [ Brain Korea 21 Center for Bio-Health Industry, and Division of Animal, Horticultural and Food Sciences, Graduate School of Chungbuk National University, Cheongju 28644, Korea ]
Damee Park [ Brain Korea 21 Center for Bio-Health Industry, and Division of Animal, Horticultural and Food Sciences, Graduate School of Chungbuk National University, Cheongju 28644, Korea ]
Tae-Jip Kim [ Brain Korea 21 Center for Bio-Health Industry, and Division of Animal, Horticultural and Food Sciences, Graduate School of Chungbuk National University, Cheongju 28644, Korea ]
본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.
간행물
간행물명
한국당과학회 학술대회
간기
연간
수록기간
2006~2022
십진분류
KDC 517DDC 614
이 권호 내 다른 논문 / 한국당과학회 학술대회 2020 한국당과학회 연례학술대회(온라인)