Earticle

초록

영어

Hemocyte parameters have been used as a proxy to characterize the health condition of marine bivalves, as the effects of external and internal stresses are reflected well in these parameters. Marine bivalve hemocyte functions are often depressed during the post-spawning period due to physiological stress and energy depletion. In this study, we analyzed temporal changes in hemocyte parameters of the oyster Saccostrea kegaki on Jeju Island, off the south coast of Korea, using flow cytometry. Total hemocyte count (THC), hemocyte types, hemocyte mortality, and phagocytosis capacity were analyzed. S. kegaki spawned during June and August, when the sea surface temperature increased from 18 to 23 °C. Most of the oysters were in the spent and resting phases from September to January. THC dropped dramatically from September to October, when most oysters completed spawning. Histology revealed that the residual eggs or sperm were actively resorbed through phagocytosis by hemocytes during the spent stage. Hemocyte mortality also showed its annual peak in October, possibly due to increased resorbing activities. The phagocytosis capacities of the granulocytes decreased dramatically from September to February. The level of energy reserves (glycogen) in post-spawning tissues was significantly lower than that prior to spawning. The low energy level reserve during the post-spawning period likely reduced the THC and immune capacities, as oysters may have been unable to acquire sufficient food from the ambient environment.

목차

ABSTRACT
1. Introduction
2. Materials and methods
2.1. Study area
2.2. Sampling effort
2.3. Hemolymph collection
2.4. Total hemocyte count and percentages of hemocyte types
2.5. Hemocyte mortality
2.6. Phagocytosis capacity
2.7. Histology
2.8. Glycogen content of tissue
2.9. Statistical analysis
3. Results
3.1. Annual gametogenesis of S. kegaki
3.2. THC and percentage of each hemocyte type
3.3. Hemocyte mortality
3.4. Phagocytosis capacity
3.5. Glycogen content of oyster tissue
4. Discussion
5. Conclusions
Credit author statement
Declaration of competing interest
Acknowledgments
References

키워드

저자

• Hyun-Ki Hong [ School of Marine Biomedical Science (BK21 PLUS), Jeju National University, Jeju 63243, Republic of Korea, Marine Science Institute, Jeju National University, Jeju 63333, Republic of Korea ]
• Kwang-Sik Choi [ School of Marine Biomedical Science (BK21 PLUS), Jeju National University, Jeju 63243, Republic of Korea, Marine Science Institute, Jeju National University, Jeju 63333, Republic of Korea ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  해양과학연구소 연구논문집 [Bulletin of the Marine Science Institute]

• 간기

  폐간

• pISSN

  1225-5734

• 수록기간

  1977~2021

• 십진분류

  KDC 454 DDC 551