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RESEARCH ARTICLE
Response of body color change rearing under different light intensity conditions in farmed red spotted grouper, Epinephelus akaara
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- 발행기관
- 제주대학교 해양과학연구소 바로가기
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- 간행물
- 해양과학연구소 연구논문집 바로가기
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- 통권
- 제44권 (2020.12)바로가기
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- 페이지
- pp.301-309
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- 저자
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- 언어
- 영어(ENG)
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- URL
- https://www.earticle.net/Article/A390100
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4,000원
원문정보
초록
- 영어
- Background: Fish body color is one of the major factors that determine the commercial value of farmed fish, to understand for coloration mechanisms. The expression of melanin-related genes is according to the developmental stage and light intensity in the red spotted grouper, Epinephelus akaara. Methods: To investigate changes in melanin formation and melanin-related genes in the larval development stage, fish larvae were reared from fertilized egg stage to 50 days after hatching (DAH). Experiment of body color changes was performed under each different light intensity conditions. Melanin-related genes expression was analyzed by real-time qPCR, and body coloration difference was represented by RGB (red, green, blue) code value. Results: Expression levels of melanin-concentrating hormone (MCH) mRNA, pro-opiomelanocortin (POMC) mRNA, and melanocortin 1 receptor (MC1R) mRNA were at their highest 5 DAH (days after hatching). Expression levels of agoutisignaling protein (ASIP) mRNA were at their highest 10 DAH. Results of body color changes according to changes in light intensity conditions showed that the expression level of MCH mRNA in the 2000 lx group was the highest at 6 weeks. The expression levels of POMC mRNA and MC1R mRNA in the 1000 lx group were at their lowest at 9 weeks. RGB color code value under different light intensities were the brightest under 1000 lx and the darkest under 0 lx. Conclusion: Our research suggests that POMC mRNA and MC1R mRNA in the red spotted grouper are involved in melanin synthesis, and these genes are thought to be controlled by light intensity. To better understand the molecular mechanism of coloration in the red spotted grouper, further studies are needed to clarify the relationship between melanophore development and melanin-related genes.
목차
Abstract
Introduction
Materials and methods
Fish
Experimental design
cDNA synthesis and real-time qPCR
RGB color code
Statistical analysis
Results
Tissue-specific expression of melanin-related genes
Developmental stages: melanin formation and melaninrelatedgenes changes
Changes in melanin-related genes expression accordingto light intensity
Discussion
Conclusion
키워드
Body coloration Melanin-concentrating hormone (MCH) Pro-opiomelanocortin (POMC) Melanocortin 1 receptor (MC1R) Agouti-signaling protein (ASIP) Red spotted grouper저자
간행물 정보
발행기관
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- 발행기관명
- 제주대학교 해양과학연구소 [Marine Science Institute Jeju National University]
- 설립연도
- 1968
- 분야
- 농수해양>수산학
- 소개
- 해양의 실체와 그 자원의 합리적 이용과 관리방법을 규명하기 위하여 해양물리, 화학, 생물, 지질, 공학등 여러 관련분야에 관한 다양한 연구를 수행하며, 환경의 보전과 보호를 위해서 수질, 대기, 상·하수도, 폐기물, 사회적 환경 등의 연구를 통하여 쾌적하고 청정한 환경을 보전하기 위한 다양한 연구를 수행하고, 또한 해양 및 환경분야의 우수한 인력양성, 국내외 학술교류의 증진, 학·산 협동체제의 확립 등을 통하여 해양과 환경에 관한 기초 및 응용적 학술연구를 종합적으로 수행함을 목적으로 한다.
간행물
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- 간행물명
- 해양과학연구소 연구논문집 [Bulletin of the Marine Science Institute]
- 간기
- 폐간
- pISSN
- 1225-5734
- 수록기간
- 1977~2021
- 십진분류
- KDC 454 DDC 551
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