Earticle

초록

영어

Galectins are well-known β-galactoside-binding proteins, which play vital roles in innate immune responses of both vertebrates and invertebrates. However, knowledge regarding invertebrate galectins is still in its infancy. With the intention of filling the knowledge gap, here we identified a quadruple domain-containing galectin from marine invertebrate disk abalone, Haliotis discus discus (AbGalec), and characterized it. AbGalec consisted of four distinct carbohydrate-recognition domains (CRDs) and lacked a signal peptide. Expression analysis revealed AbGalec to be ubiquitously expressed in all the examined early embryonic stages of abalone, with highest expression in the 16-cell stage, suggesting the importance of AbGalec in early developmental processes. Tissue distribution analysis revealed the highest expression of AbGalec in abalone mantle, followed by that in gills and hemocytes. Immune challenge experiments revealed significant upregulation of AbGalec at 24 h and 48 h post injection (p.i.) with bacterial and viral components. These results suggested the possible involvement of AbGalec in host defense mechanisms. Polyinosinic: polycytidylic acid (Poly I:C) and viral hemorrhagic septicemia virus (VHSV) injections were capable of inducing AbGalec transcript expression more prominently than bacterial stimulants, thus providing evidence for its role in viral infections. We determined the virus-neutralizing ability of a quadruple domain-containing galectin for the first time, by analyzing the downregulation of VHSV transcripts during the overexpression of AbGalec. Significant downregulation of VHSV transcripts was observed after 24 h and 48 h of post infection. Collectively, our findings reveal the potent antiviral responses of molluscan quadruple domain-containing galectin, AbGalec, along with its involvement in innate immunity.

목차

ABSTRACT
1. Introduction
2. Methodology
2.1. Experimental animal husbandry
2.2. Collection of samples from different developmental stages of abalones
2.3. Tissue sampling and in-vivo immune challenge experiment
2.4. RNA extraction and cDNA synthesis
2.5. Molecular and genomic characterization of AbGalec
2.6. Transcriptional analysis of AbGalec
2.7. Construction of expression plasmids
2.8. Cell culture and transfection
2.9. Antiviral activity assay
2.10. Statistical analysis
3. Results
3.1. Identification and sequence analysis of AbGalec
3.2. Homology analysis of AbGalec
3.3. Localization of AbGalec in disk abalone tissues
3.4. Expression of AbGalec at different stages of disk abalonedevelopment
3.5. Immune challenge experiment
3.6. Reduction of viral transcription by AbGalec
4. Discussion
5. Conclusion
CRediT authorship contribution statement
Acknowledgement
References

키워드

저자

• W.M. Gayashani Sandamalika [ Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National University, Jeju Self-Governing Province, 63243, Republic of Korea, Marine Science Institute, Jeju National University, Jeju Self-Governing Province, 63333, Republic of Korea ]
• Jehee Lee [ Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National University, Jeju Self-Governing Province, 63243, Republic of Korea, Marine Science Institute, Jeju National University, Jeju Self-Governing Province, 63333, Republic of Korea ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  해양과학연구소 연구논문집 [Bulletin of the Marine Science Institute]

• 간기

  폐간

• pISSN

  1225-5734

• 수록기간

  1977~2021

• 십진분류

  KDC 454 DDC 551