Molecular characterization of Urosporidium tapetis sp. nov., a haplosporidian hyperparasite infecting metacercariae of Parvatrema duboisi (Dollfus 1923), a trematode parasite of Manila clam Ruditapes philippinarum on the west coast of Korea
Young-Ghan Cho, Hyun-Sil Kang, Cuong Thanh Le, Mun Gyeong Kwon, Min-Seok Jang, Kwang-Sik Choi
언어
영어(ENG)
URL
https://www.earticle.net/Article/A390091
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초록
영어
Recently, a putative new hyperparasitic haplosporidian in the genus Urosporidium was identified from metacercariae of the trematode Parvatrema duboisi infecting Manila clam Ruditapes philippinarum on the west coast of Korea. In this study, we applied small subunit ribosomal DNA (SSU rDNA) sequences as a marker to substantiate the phylogenetic relationship of the unidentified Urosporidium within the Order Haplosporida. In our phylogenetic analysis, the 1890 bp of SSU rDNA sequences obtained were closely related to a haplosporidian parasite forming a sister clade to Urosporidium group, although the gene sequences were only 89.22–89.70% similar to Urosporidium spp. Such molecular phylogenetic distance within the genus suggested that the unidentified Urosporidium is a new member of the genus. Accordingly, we report the unidentified haplosporidian hyperparasite as Urosporidium tapetis sp. nov.
목차
ABSTRACT 1. Introduction 2. Materials and methods 2.1. Clam sampling and spore isolation from the host 2.2. Scanning electron microscopy (SEM) 2.3. DNA extraction, amplification, and sequencing of Urosporidium sp. 3. Results and discussion 3.1. Spore isolation and SEM 3.2. DNA sequence and phylogenetic analysis Acknowledgments References
Young-Ghan Cho [ Department of Marine Life Science (BK21 PLUS) and Marine Science Institute, Jeju National University, 102 Jejudaehakno, Jeju 63243, Republic of Korea ]
Hyun-Sil Kang [ Department of Marine Life Science (BK21 PLUS) and Marine Science Institute, Jeju National University, 102 Jejudaehakno, Jeju 63243, Republic of Korea, Southeast Sea Fisheries Research Institute, National Institute of Fisheries Science (NIFS) of Korea, Tongyeong 53085, Republic of Korea ]
Cuong Thanh Le [ Department of Marine Life Science (BK21 PLUS) and Marine Science Institute, Jeju National University, 102 Jejudaehakno, Jeju 63243, Republic of Korea, Institute for Aquaculture, Nha Trang University, 02 Nguyen Dinh Chieu, Nhatrang, Viet Nam ]
Mun Gyeong Kwon [ Aquatic Disease Control Division, National Institute of Fisheries Science (NIFS), Busan 46083, Republic of Korea ]
Min-Seok Jang [ South Sea Fisheries Research Institute, National Institute of Fisheries Science (NIFS), Yeosu 59780, Republic of Korea ]
Kwang-Sik Choi [ Department of Marine Life Science (BK21 PLUS) and Marine Science Institute, Jeju National University, 102 Jejudaehakno, Jeju 63243, Republic of Korea ]
Corresponding Author
제주대학교 해양과학연구소 [Marine Science Institute Jeju National University]
설립연도
1968
분야
농수해양>수산학
소개
해양의 실체와 그 자원의 합리적 이용과 관리방법을 규명하기 위하여 해양물리, 화학, 생물, 지질, 공학등 여러 관련분야에 관한 다양한 연구를 수행하며, 환경의 보전과 보호를 위해서 수질, 대기, 상·하수도, 폐기물, 사회적 환경 등의 연구를 통하여 쾌적하고 청정한 환경을 보전하기 위한 다양한 연구를 수행하고, 또한 해양 및 환경분야의 우수한 인력양성, 국내외 학술교류의 증진, 학·산 협동체제의 확립 등을 통하여 해양과 환경에 관한 기초 및 응용적 학술연구를 종합적으로 수행함을 목적으로 한다.
간행물
간행물명
해양과학연구소 연구논문집 [Bulletin of the Marine Science Institute]