Earticle

초록

영어

The previous study suggested that the sulfated polysaccharides from Hizikia fusiforme (HFPS) possess strong antioxidant activity. The purpose of this study is to isolate fucoidan from HFPS and to investigate its antioxidant activity. Afucoidan (HFPS-F4) with a molecular weight of 102.67 kDa was isolated from HFPS. HFPS-F4 contains 99.01% of fucoidan (71.79 ± 0.56% of carbohydrate and 27.22 ± 0.05% of sulfate content). The fucoidan increased the viability of H2O2-treated Vero cells by 5.41, 11.17, and 16.32% at the concentration of 12.5, 25, and 50 μg/mL, respectively. Further results demonstrated that this effect act diminishing apoptosis by scavenging intracellular reactive oxygen species (ROS) via increasing the expression of the endogenous antioxidant enzymes, which was induced by elevating total nuclear factor (erythroid-derived 2)-like 2 (Nrf2) levels. In addition, the in vivo test results displayed that the pretreatment of fucoidan improved the survival rates and decreased heart-beating rate, ROS, cell death, and lipid peroxidation in H2O2-stimulated zebrafish. Taken together, these results demonstrated that fucoidan isolated from HFPS has strong in vitro and in vivo antioxidant activities and it could be utilized in pharmaceutical, nutraceutical, and cosmeceutical industries.

목차

Abstract
1. Introduction
2. Materials and Methods
2.1. Reagents and Chemicals
2.2. Extraction and Isolation
2.3. Free Radical Scavenging Activity
2.4. Chemical Composition Analysis
2.5. Chemical Characterization of HFPS-F4
2.6. Cell Culture
2.7. Effect of Fucoidan on H2O2-Stimulated Vero Cells
2.8. Effect of Fucoidan on H2O2-Stimulated Zebrafish
2.9. Statistical Analysis
3. Results and Discussion
3.1. Free Radical Scavenging Activities of the Polysaccharides Isolated from HFPS
3.2. Chemical Composition and Structural Characteristics of HFPS-F4
3.3. In Vitro Antioxidant Activity of Fucoidan
3.4. In Vivo Antioxidant Activity of Fucoidan
4. Conclusions
References

키워드

저자

• Lei Wang [ Department of Marine Life Sciences, Jeju National University, Jeju, Marine Science Institute, Jeju National University, Jeju, Jeju Self-Governing Province 63333, Korea ]
• Thilina U. Jayawardena [ Department of Marine Life Sciences, Jeju National University, Jeju, Jeju Self-Governing Province 63243, Korea ]
• Hye-Won Yang [ Department of Marine Life Sciences, Jeju National University, Jeju, Jeju Self-Governing Province 63243, Korea ]
• Hyo Geun Lee [ Department of Marine Life Sciences, Jeju National University, Jeju, Jeju Self-Governing Province 63243, Korea ]
• Min-Cheol Kang [ Department of Marine Life Sciences, Jeju National University, Jeju, Jeju Self-Governing Province 63243, Korea, Research Group of Process Engineering, Korea Food Research Institute, Wanju, Jeollabuk-do 55365, Korea ]
• K. K. Asanka Sanjeewa [ Department of Marine Life Sciences, Jeju National University, Jeju, Jeju Self-Governing Province 63243, Korea ]
• Jae Young Oh [ Department of Marine Life Sciences, Jeju National University, Jeju, Jeju Self-Governing Province 63243, Korea ] Corresponding Author
• You-Jin Jeon [ Department of Marine Life Sciences, Jeju National University, Jeju, Marine Science Institute, Jeju National University, Jeju, Jeju Self-Governing Province 63333, Korea ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  해양과학연구소 연구논문집 [Bulletin of the Marine Science Institute]

• 간기

  폐간

• pISSN

  1225-5734

• 수록기간

  1977~2021

• 십진분류

  KDC 454 DDC 551