Earticle

초록

영어

Leptin, a well-known appetite hormone, plays a role in fat metabolism in peripheral tissues including the adipose, liver, and muscle tissues. In this study, we investigated the antiobesity and fat accumulation regulatory effects of Ishophloroglucin A, derived from the brown seaweed Ishige okamurae, which acts via the leptin signaling pathway in the peripheral tissues of a high-fat-diet-induced obese mouse model. Obesity in C57BL/6J mice was induced by feeding them with a high-fat diet for 10 weeks and Ishophloroglucin A (2.5 mg/kg) was orally treated for the last 4 weeks. Body weights were monitored once per week during the experimental period. After the experiment, several serum biochemical parameters were measured using commercial kits and the white adipose, liver, and muscle tissues were observed using immunohistochemistry methods. Ishophloroglucin A significantly reduced glutamic oxaloacetic transaminase, glutamic pyruvic transaminase, and leptin level, which increase as a result of high-fat diet. Also, Ishophloroglucin A clearly activated the leptin signaling pathway in all examined peripheral tissues, reduced the adipose tissue size, and alleviated steatosis in the liver and muscle tissues. These results implied that Ishophloroglucin A treatment for 4 weeks positively induced molecular mechanisms and histologic changes related with leptin signaling. These findings suggested that constant Ishophloroglucin A treatment clearly regulates obesity and peripheral fat accumulation via the leptin signaling pathway in high-fat-diet-induced obese mice.

목차

ABSTRACT
1. Introduction
2. Materials and methods
2.1. Isolation of Ishophloroglucin (IPA)
2.2. Animal experiment
2.3. Signal analysis
2.4. Statistical analysis
3. Results and discussion
3.1. Antiobesity effects of Ishophloroglucin (IPA) in high-fat-diet-inducedobese C57BL/6J mice
3.2. Effects of Ishophloroglucin (IPA) on peripheral metabolism via theleptin signaling pathway
3.3. Effects of Ishophloroglucin (IPA) on fat expansion and accumulation inperipheral organs
4. Conclusion
Statement of informed consent, human/animal rights
CRediT authorship contribution statement
Declaration of competing interest
Acknowledgments
Appendix A. Supplementary data
References

키워드

저자

• Nalae Kang [ Jeju Marine Research Center, Korea Institute of Ocean Science and Technology (KIOST), Jeju 63349, Republic of Korea,Department of Marine Life Sciences, Jeju National University, Jeju 63243, Republic of Korea, ]
• Seyeon Oh [ Functional Cellular Networks Laboratory, College of Medicine, Department of Medicine, Graduate School and Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Incheon 21999, Republic of Korea ]
• Hyun-Soo Kim [ National Marine Biodiversity Institute of Korea, Seocheon 33662, Republic of Korea ]
• Hyosang Ahn [ Functional Cellular Networks Laboratory, College of Medicine, Department of Medicine, Graduate School and Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Incheon 21999, Republic of Korea ]
• Junwon Choi [ Functional Cellular Networks Laboratory, College of Medicine, Department of Medicine, Graduate School and Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Incheon 21999, Republic of Korea, Department of Anatomy & Cell biology, Gachon University College of Medicine, Incheon 21936, Republic of Korea ]
• Soo-Jin Heo [ Jeju Marine Research Center, Korea Institute of Ocean Science and Technology (KIOST), Jeju 63349, Republic of Korea, Department of Biology, University of Science and Technology (UST), Daejeon 34113, Republic of Korea ]
• Kyunghee Byun [ Functional Cellular Networks Laboratory, College of Medicine, Department of Medicine, Graduate School and Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Incheon 21999, Republic of Korea, Department of Anatomy & Cell biology, Gachon University College of Medicine, Incheon 21936, Republic of Korea ] Corresponding Author
• You-Jin Jeon [ Department of Marine Life Sciences, Jeju National University, Jeju 63243, Republic of Korea, Marine Science Institute, Jeju National University, Jeju 63333, Republic of Korea ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  해양과학연구소 연구논문집 [Bulletin of the Marine Science Institute]

• 간기

  폐간

• pISSN

  1225-5734

• 수록기간

  1977~2021

• 십진분류

  KDC 454 DDC 551