Characterization and expression analysis of rockfish (Sebastes schtegeIii) myeloid differentiation factor-88 (SsMyD88) and evaluation of its ability to induce inflammatory cytokines through NF-KB
ABSTRACT 1. Introduction 2. Materials and Methods 2.1 Identification of MyD88 from rockfish and in silico analysis 2.2 Fish rearing 2.3 Tissue/organ sampling for assays of tissue distribution and gene expression upon immune challenge 2.4 Construction of the expression plasmid 2.5 Cell culture and transfection 2.6 Inflammatory gene expression assay 2.7 RNA extraction and cDNA synthesis 2.8 Quantitative real-time PCR(qPCR) 2.9 Luciferase assay 2.10 NO Production and F-action fluorescent detection 2.11 Statistical analysis 3. Results 3.1 In silico analysis of SsMyD88 3.2 Unchallenged spatial expression pattern of SsMyD88 in vivo 3.3 Immune stimulants and temporal fluctuation of SsMyD88 in vivo 3.4 Downstream promoter biases for SsMyD88 3.5 SsMyD88 induces inflammatory activation 4. Discussion 5. Conclusion Acknowledgments References
K.A.S.N. Shanaka [ Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National University, Jeju Self-Governing Province ]
M.D. Neranjan Tharuka [ Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National University, Jeju Self-Governing Province ]
Sarithaa Sellaththurai [ Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National University, Jeju Self-Governing Province ]
Hyerim Yang [ Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National University, Jeju Self-Governing Province ]
Thanthrige Thiunuwan Priyathilaka [ Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National University, Jeju Self-Governing Province ]
Jehee Lee [ Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National University, Jeju Self-Governing Province, Marine Science Institute, Jeju National University, Jeju Self-Governing Province, 63333, Republic of Korea ]
Corresponding Author
제주대학교 해양과학연구소 [Marine Science Institute Jeju National University]
설립연도
1968
분야
농수해양>수산학
소개
해양의 실체와 그 자원의 합리적 이용과 관리방법을 규명하기 위하여 해양물리, 화학, 생물, 지질, 공학등 여러 관련분야에 관한 다양한 연구를 수행하며, 환경의 보전과 보호를 위해서 수질, 대기, 상·하수도, 폐기물, 사회적 환경 등의 연구를 통하여 쾌적하고 청정한 환경을 보전하기 위한 다양한 연구를 수행하고, 또한 해양 및 환경분야의 우수한 인력양성, 국내외 학술교류의 증진, 학·산 협동체제의 확립 등을 통하여 해양과 환경에 관한 기초 및 응용적 학술연구를 종합적으로 수행함을 목적으로 한다.
간행물
간행물명
해양과학연구소 연구논문집 [Bulletin of the Marine Science Institute]