Earticle

초록

영어

In this study, the saponin-rich fractions of five individual (two Red and three Black) sea cucumbers (Apostichopus japonicus) in South Korea were investigated for their antiproliferative effect against HL-60, B16F10, MCF-7, and Hep3B tumor cell lines. The red sea cucumber saponin-rich fraction (SSC) from Jeju Island (JRe) decreased the growth of HL-60 with an IC50 value of 23.55 ± 3.40 lg/mL, which represented the strongest anticancer activity among the extracts. Further, SSC downregulated B-cell lymphoma extra-large (Bcl-xL), while upregulating, to different degrees, Bcl-2-associated X protein (Bax), caspase-9, caspase-3, PARP cleavage, and apoptotic bodies in cancer cells. Evidence for SSC inducing apoptosis via the mitochondria-mediated pathway was found. The contents of SSCs were determined using ultra high-performance liquid chromatography coupled with a quadrupole orbitrap mass spectrometry to comparatively evaluate the regional influence. In West Sea, the total SSC content of A. japonicus was 15.5 mg/g, representing the highest content, while A. japonicus in the South Sea yielded the lowest content at 8 mg/g. The major saponin constituent in SSC was identified as Holotoxin A1, which may the anti-tumor compound in A. japonicus.

목차

Abstract
Introduction
Material and methods
Materials and apparatus
Collection of sea cucumber
Preparation of SSCs
Semi-quantitative determination of SSCs
Ultra-high-performance liquid chromatography equipped with a quadrupole orbitrap mass spectrometer (UHPLC/Q-Orbitrap MS) analysis
Cell culture
Apoptosis analysis by flow cytometry
Apoptotic and necrotic body formation
Western blot analysis
Statistical analysis
Results and discussion
Proximate compositions of A. japonicus
Semi-quantitative determination and UHPLC/QOrbitrapMS analysis of SSCs
Cytotoxicity and anticancer effects of SSCs
Apoptosis morphology of HL-60 cell line due to JRe and WBa
Apoptosis pathway regulated by JRe
Conclusion
References

키워드

저자

• Yu-Lin Dai [ Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, China, Department of Marine Life Science, Jeju National University, Jeju 63243, Republic of Korea ]
• Eun-A Kim [ Jeju Research Institute, Korea Institute of Ocean Science & Technology (KIOST), Jeju 63349, Republic of Korea ]
• Hao-Ming Luo [ School of Pharmacy, Changchun University of Chinese Medicine, Changchun 130117, China ]
• Yun-Fei Jiang [ Department of Marine Life Science, Jeju National University, Jeju 63243, Republic of Korea ]
• Jae-Young Oh [ Department of Marine Life Science, Jeju National University, Jeju 63243, Republic of Korea ]
• Soo-Jin Heo [ Jeju Research Institute, Korea Institute of Ocean Science & Technology (KIOST), Jeju 63349, Republic of Korea ]
• You-Jin Jeon [ Department of Marine Life Science, Jeju National University, Jeju 63243, Republic of Korea, Marine Science Institute, Jeju National University, Jeju 63333, Republic of Korea ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  해양과학연구소 연구논문집 [Bulletin of the Marine Science Institute]

• 간기

  폐간

• pISSN

  1225-5734

• 수록기간

  1977~2021

• 십진분류

  KDC 454 DDC 551