Original Article
Alpha-linolenic acid enhances maturation and developmental competence via regulation of glutathione, cAMP and fatty acid accumulation during in vitro maturation of porcine oocytes
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- 발행기관
- 한국동물생명공학회(구 한국동물번식학회) 바로가기
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- 간행물
- Journal of Animal Reproduction and Biotechnology 바로가기
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- 통권
- Volume. 35 No. 4 (2020.12)바로가기
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- 페이지
- pp.357-365
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- 저자
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- 언어
- 영어(ENG)
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- URL
- https://www.earticle.net/Article/A388202
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원문정보
초록
- 영어
- The aim of present study was to investigate regulatory mechanism of alpha-linolenic acid (ALA) during in vitro maturation (IVM) on nuclear and cytoplasmic maturation of porcine oocytes. Basically, immature cumulus-oocyte complexes (COCs) were incubated for 22 h in IVM-I to which hormone was added, and then further incubated for 22 h in IVM-II without hormone. As a result, relative cumulus expansion was increased at 22 h after IVM and it was enhanced by treatment of ALA compared with control group (p < 0.05). During IVM process within 22 h, cAMP level in oocytes was decreased at 6 h (p < 0.05) and it was recovered at 12 h in ALA-treated group, while oocytes in control group recovered cAMP level at 22 h. In cumulus cells, it was reduced in all time point (p < 0.05) and ALA did not affect. Treatment of ALA enhanced metaphase-I (MI) and MII population of oocytes compared with oocytes in control group at 22 and 44 h, respectively (p < 0.05). Intracellular GSH levels in ALA group was increased at 22 and 44 h after IVM (p < 0.05), whereas it was increased in control group at 44 h after IVM (p < 0.05). In particular, the GSH in ALA-treated oocytes during 22 h of IVM was higher than control group at 22 h (p < 0.05). Lipid amount in oocytes from ALA group was higher than control group (p < 0.05). Treatment of ALA did not influence to absorption of glucose from medium. Cleavage and blastocyst formation of ALA-treated oocytes were enhanced compared with control group (p < 0.05). These findings suggest that supplementation of ALA could improve oocyte maturation and development competence through increasing GSH synthesis, lipid storage, and regulation of cAMP accumulation during early 22 h of IVM, and these might be mediated by cumulus expansion.
목차
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Chemicals
In vitro maturation
Assessment of cumulus expansion
Measurement of cAMP concentration
Evaluation of nuclear maturation
Measurement of intracellular GSH level
Staining of lipid droplet
Glucose absorption
In vitro fertilization and culture
Statistical analysis
RESULTS
Changes of cumulus expansion and cAMP level during first 22 h of IVM by ALA treatment
Effect of ALA on nuclear maturation at 22 and 44 h after IVM
Intracellular GSH level and fatty acid content
Change of glucose level in maturation medium and embryonic development by ALA
DISCUSSION
CONFLICTS OF INTEREST
ACKNOWLEDGEMENTS
AUTHOR CONTRIBUTIONS
AUTHOR’S POSITION AND ORCID NO.
REFERENCES
키워드
alpha-linolenic acid cyclic adenosine monophosphate glutathione In vitro maturation porcine oocytes저자
간행물 정보
발행기관
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- 발행기관명
- 한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
- 설립연도
- 1976
- 분야
- 농수해양>축산학
- 소개
- 동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.
간행물
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- 간행물명
- Journal of Animal Reproduction and Biotechnology
- 간기
- 계간
- pISSN
- 2671-4639
- eISSN
- 2671-4663
- 수록기간
- 2019~2026
- 십진분류
- KDC 527 DDC 636
이 권호 내 다른 논문 / Journal of Animal Reproduction and Biotechnology Volume. 35 No. 4
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