Earticle

초록

영어

Cytochrome P450 1A2 (CYP1A2) is a member of the cytochrome P450 superfamily enzymes in mammals and plays a major role in metabolizing endogenous hormones in the liver. In recent days, CYP1A2 expression has been found in not only the liver but also other tissues including the pancreas and lung. However, little information is available regarding the expression of CYP1A2 in the ovary, in spite of the facts that the ovarian follicle growth and atresia are tightly associated with controls of endocrine hormonal networks. Therefore, the expression of CYP1A2 in the ovaries of prepubertal and pubertal rats was investigated to assess its expression pattern and puberty-related alteration. It was demonstrated that the expression level of CYP1A2 was significantly (p < 0.01) higher in the pubertal ovaries than prepubertal counterparts. At the ovarian follicle level in both groups, whereas CYP1A2 expression was less detectable in the primordial, primary and secondary follicles, the strongly positive expression of CYP1A2 was localized in the granulosa cell layers in the antral and pre-ovulatory follicles. However, the ratio of CYP1A2-positive ovarian follicle was significantly (p < 0.01) higher in the ovary of pubertal group (73.1 ± 3.1%) than prepubertal one (41.0 ± 10.5%). During the Immunofluorescence, expression of CYP1A2 was mainly localized in Fas-positive follicles, indicating the atretic follicles. In conclusion, these results suggested that CYP1A2 expression was mainly localized at the atretic follicular cells and affected by the onset of puberty. Further study is still necessary but we hypothesize that CYP1A2 expresses in the atretic follicles to metabolize residue of the reproductive hormones. These findings may have important implications for the fields of reproductive biology of animals.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Ethics statement
Chemicals and media
Animal housing and experiment groups
Sacrifice of animals and sampling
Body weight and vaginal opening assessment
Western blotting for quantitative expression of CYP1A2
Hematoxylin & eosin staining and immunohistochemistry
Classification of ovarian follicles
Immunofluorescence
Statistical analysis
RESULTS
Confirmation of prepuberty and puberty
Western blotting for quantitative assessment of CYP1A2 expression
Immunohistochemistry of CYP1A2 in prepubertal and pubertal ovaries
Immunofluorescence of CYP1A2 and Fas in the ovary
DISCUSSION
CONFLICTS OF INTEREST
CKNOWLEDGEMENTS
AUTHOR CONTRIBUTIONS
AUTHOR’S POSITION AND ORCID NO.
REFERENCES

키워드

저자

• Jong-Chan Hwang [ College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Korea ]
• Byung-Joon Park [ College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Korea ]
• Hwan-Deuk Kim [ College of Veterinary Medicine, Kyungpook National University, Department of Veterinary Research, Daegu Metropolitan City Institute of Health & Environment, Daegu 42183, Korea ]
• Su-Min Baek [ College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Korea ]
• Seoung-Woo Lee [ College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Korea ]
• Ryoung-Hoon Jeon [ College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea ]
• Min Jang [ College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Korea ]
• Seul-Gi Bae [ College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Korea ]
• Sung-Ho Yun [ College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Korea ]
• Jin-Kyu Park [ College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Korea ]
• Young-Sam Kwon [ College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Korea ]
• Seung-Joon Kim [ College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Korea ]
• Won-Jae Lee [ College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Korea ] Corresponding Author

참고문헌

발행기관

간행물

관심저널 등록

• 간행물명

  Journal of Animal Reproduction and Biotechnology

• 간기

  계간

• pISSN

  2671-4639

• eISSN

  2671-4663

• 수록기간

  2019~2026

• 십진분류

  KDC 527 DDC 636