Earticle

초록

영어

In the present study, we examined the effect of straw size on spermatozoa motility, viability, acrosome integrity, mitochondrial membrane potential, and plasma membrane integrity after freezing-thawing. Hanwoo semen was collected from three bulls and diluted with an animal protein-free extender, divided into two groups, namely, 10 million spermatozoa in 0.25 mL and 20 million spermatozoa in 0.5 mL straw, and cryopreserved. In Experiment 1, the motility and motility parameters of the frozenthawed spermatozoa were evaluated. After freezing-thawing, the spermatozoa motility parameters fast progressive, straight line velocity, and average path velocity were compared between the 0.25 mL straw and 0.5 mL straw groups. They were 35.2 ± 1.0 and 32.3 ± 0.7%, 34.6 ± 0.7 and 31.8 ± 0.5 μm/s, 51.4 ± 1.3 and 47.1 ± 1.1 μm/s, 0.25 mL straw and 0.5 mL straw groups, respectively. In Experiment 2, the viability, acrosome membrane integrity, and mitochondrial membrane potential of the frozen-thawed spermatozoa were assessed. After freezing-thawing, the percentages of spermatozoa with live, intact acrosomes and high mitochondrial membrane potential were compared between the in 0.25 mL straw and 0.5 mL straw groups. They were 48.0 ± 2.6% and 35.6 ± 2.8% between the 0.25 mL straw and 0.5 mL straw groups. In Experiment 3, the plasma membrane integrity of frozen-thawed spermatozoa was compared. After freezingthawing, the plasma membrane integrity was higher for the in 0.25 mL straw group than the 0.5 mL straw group. They were 62.0 ± 2.2 and 54.1 ± 1.3% between the 0.25 mL straw and 0.5 mL straw groups. In conclusion, our results suggest that freezing semen in 0.25 mL straw improves the relative motility, viability, and acrosomal, mitochondrial membrane potential, and plasma membrane integrity of Hanwoo bull spermatozoa.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Bull semen collection and cryopreservation
Spermatozoa motility and motility parameters after freezing-thawing
Spermatozoa viability, acrosomal membrane integrity, and mitochondrial membrane potential of after freezing-thawing
Fluorescence preparation
Spermatozoa plasma membrane integrity after freezing-thawing
Statistical analysis
RESULTS
Experiment 1
Experiment 2
Experiment 3
DISCUSSION
CONCLUSION
CONFLICTS OF INTEREST
ACKNOWLEDGEMENTS
AUTHOR CONTRIBUTIONS
AUTHOR’S POSITION AND ORCID NO.
REFERENCES

키워드

저자

• Sung-Sik Kang [ Hanwoo Research Institute, National Institute Animal Science (NIAS), Rural Development Administration (RDA), Pyeongchang 25340, Korea ]
• Ui-Hyung Kim [ Hanwoo Research Institute, National Institute Animal Science (NIAS), Rural Development Administration (RDA), Pyeongchang 25340, Korea ]
• Myung-Suk Lee [ Hanwoo Research Institute, National Institute Animal Science (NIAS), Rural Development Administration (RDA), Pyeongchang 25340, Korea ]
• Seok-Dong Lee [ Hanwoo Research Institute, National Institute Animal Science (NIAS), Rural Development Administration (RDA), Pyeongchang 25340, Korea ]
• Sang-Rae Cho [ Hanwoo Research Institute, National Institute Animal Science (NIAS), Rural Development Administration (RDA), Pyeongchang 25340, Korea ] Corresponding Author

참고문헌

발행기관

간행물

관심저널 등록

• 간행물명

  Journal of Animal Reproduction and Biotechnology

• 간기

  계간

• pISSN

  2671-4639

• eISSN

  2671-4663

• 수록기간

  2019~2026

• 십진분류

  KDC 527 DDC 636