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Original Article

Expression profile of spermatogenesis associated genes in male germ cells during postnatal development in mice

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  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Journal of Animal Reproduction and Biotechnology 바로가기
  • 통권
    Volume. 35 No. 4 (2020.12)바로가기
  • 페이지
    pp.289-296
  • 저자
    Jin Seop Ahn, Hyun-Sung Ryu, Sang-Eun Jung, Beom-Jin Shin, Jong-Hyun Won, Tea Gun Um, Huijo Oh, Seo-Hee Kim, Buom-Yong Ryu
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A388194

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원문정보

초록

영어
Spermatogonial stem cells are self-renewal and differentiate into sperm in post-pubertal mammals. There exists a balance between the self-renewal and differentiation in the testes. Spermatogonial stem cells make up only 0.03% of testicular cells in adult mice. These cells maintain sperm production by differentiating after puberty. Therefore, analyzing the expression of genes associated with spermatogenesis is critical for understanding differentiation. The present study aimed to establish the postnatal period of cells in relation to spermatogenesis. To study the expression of differentiated and undifferentiated marker genes in enriched spermatogonial stem cells, in vitro culture was performed and cells from pup (6–8-day-old) and adult (4-months-old) testicular tissues were isolated. As a result, undifferentiated genes, Pax7, Plzf, GFRa1, Etv5 and Bcl6b , were highly increased in cultured spermaotogonial stem cells compared with pup and adult testicular cells. On the other hands, differentiated gene, c-kit was highly increased in adult testicular cells, Also Stra8 gene was highly increased in pup and adult testicular cells. This study provides a better understanding of spermatogenesis-associated gene expression during postnatal periods.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Animals
Isolation and culture of mouse spermatogonial stem cells
Isolation of testicular cells
Hematoxylin & eosin staining
Immunohistochemistry
Quantitative reverse transcription polymerase chain reaction (qRT-PCR)
Statistical analysis
RESULTS
Identification of germ cells in mouse testes
Differential expression of undifferentiated spermatogonial stem cell-associated genes
Differential expression of differentiated spermatogonial stem cell-associated genes
DISCUSSION
CONFLICTS OF INTEREST
AUTHOR CONTRIBUTIONS
AUTHOR’S POSITION AND ORCID NO.
REFERENCES

키워드

adult testicular cells gene expression pup testicular cells spermatogenesis spermatogonial stem cells

저자

  • Jin Seop Ahn [ Department of Animal Science and Technology, Chung Ang University, Anseong, 17546, Korea ]
  • Hyun-Sung Ryu [ Department of Biosciences, Durham University, Durham DH1 3LE, UK ]
  • Sang-Eun Jung [ Department of Animal Science and Technology, Chung Ang University, Anseong, 17546, Korea ]
  • Beom-Jin Shin [ Department of Animal Science and Technology, Chung Ang University, Anseong, 17546, Korea ]
  • Jong-Hyun Won [ Department of Animal Science and Technology, Chung Ang University, Anseong, 17546, Korea ]
  • Tea Gun Um [ Department of Animal Science and Technology, Chung Ang University, Anseong, 17546, Korea ]
  • Huijo Oh [ Department of Animal Science and Technology, Chung Ang University, Anseong, 17546, Korea ]
  • Seo-Hee Kim [ Department of Animal Science and Technology, Chung Ang University, Anseong, 17546, Korea ]
  • Buom-Yong Ryu [ Department of Animal Science and Technology, Chung Ang University, Anseong, 17546, Korea ] Corresponding Author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Journal of Animal Reproduction and Biotechnology
  • 간기
    계간
  • pISSN
    2671-4639
  • eISSN
    2671-4663
  • 수록기간
    2019~2025
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / Journal of Animal Reproduction and Biotechnology Volume. 35 No. 4

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