Earticle

초록

영어

As the importance of biological resources increases, the conservation technology is becoming important for rarities. This study was conducted to establish an efficient cryopreservation conditions for the Deutzia paniculata, endangered plant species, by using both cryopreservation methods of vitrification and encapsulation. As a result, the sucrose pretreatment seed viability showed up to 30.7% in the treatments. The cryoprotectant treatment improved the viability of the seeds, and was found to be excellent in the vitrification method using PVS3. The vitrification method had over 10% higher germination rate than the seeds preserved by encapsulation. In addition, the germination rate showed a significant difference according to the cryopreservation treatment time, and the germination rate of seeds decreased very much as the long time became longer. Plants germinated from preserved seed in liquid nitrogen showed poor growth compared to untreated, and good growth in PVS3 120 minutes. In addition, the growth of germinated plants by liquid nitrogen treatment time was better in the vitrification method. These results are expected to be useful for long-term preservation of D. paniculata, endangered plants.

목차

Abstract
Introduction
Materials and Methods
Plant materials and cultivation
Sucrose pretreatment
Encapsulation method
Vitrification method
Thawing and plant formation
Measurement of seed viability
Measurement of germination rate
Growth and acclimation of the in vitro seedlings
Statistical analysis
Results
Seed viability by sucrose pretreatment
Germination rate by cryopreservation method
Acclimatization and growth of seedlings cryopreserved using LN
Discussion
References

키워드

저자

• Yuwon Seol [ Division of Environmental Forest Science, Gyeongsang National University (Institute of Agriculture of Life Science), Jinju 52828, Republic of Korea ]
• Seong Hyeon Yong [ Division of Environmental Forest Science, Gyeongsang National University (Institute of Agriculture of Life Science), Jinju 52828, Republic of Korea ]
• Eunji Choi [ Division of Environmental Forest Science, Gyeongsang National University (Institute of Agriculture of Life Science), Jinju 52828, Republic of Korea ]
• Mi Jin Jeong [ Division of Plant Resources, Korea National Arboretum, Yangpyeong 12519, Republic of Korea ]
• Gang Uk Suh [ Division of Plant Resources, Korea National Arboretum, Yangpyeong 12519, Republic of Korea ]
• Cheul Ho Lee [ DMZ Botanic Garden, Korea National Arboretum, Yanggu 24564, Republic of Korea ]
• Myung Suk Choi [ Division of Environmental Forest Science, Gyeongsang National University (Institute of Agriculture of Life Science), Jinju 52828, Republic of Korea ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Journal of Forest and Environmental Science [산림과학연구]

• 간기

  계간

• pISSN

  2288-9744

• eISSN

  2288-9752

• 수록기간

  1981~2026

• 등재여부

  KCI 등재

• 십진분류

  KDC 526 DDC 634