Feriel Yasmine Mahiddine, Ahmad Yar Qamar, Min Jung Kim
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영어(ENG)
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https://www.earticle.net/Article/A382679
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초록
영어
Amniotic membrane stem cells are considered as a good alternative to embryonic stem cells, but their use in clinical studies is still not common. Here, exosomes from canine amniotic membrane mesenchymal stem cells (cAmMSCexo) were used for dog sperm cryopreservation. Upon cryopreserved straws using cryoprotectant containing 0, 0.5, 1, or 2 μg/mL of cAmMSC-exo were thawed, motility and membrane integrity were analyzed. However, results showed no significant differences between the groups. We concluded that cAmMSC-exo with lower than 2 µg/mL have no effects on sperm cryopreservation, and further studies to get higher concentrations of cAmMSC-exo should be conducted for clinical application.
목차
ABSTRACT INTRODUCTION MATERIEL AND METHODS Cell culture and exosomes extraction Animal use and semen processing Cryopreservation and thawing process Sperm parameters evaluation RESULTS AND DISCUSSION CONCLUSION CONFLICTS OF INTEREST ACKNOWLEDGEMENTS AUTHOR CONTRIBUTIONS AUTHOR’S POSITION AND ORCID NO. REFERENCES
Feriel Yasmine Mahiddine [ Department of Theriogenology and Biotechnologies, College of Veterinary Medicine, Seoul National University ]
Ahmad Yar Qamar [ Laboratory of Theriogenology, College of Veterinary Medicine, Chungnam National University, Department of Clinical Sciences, College of Veterinary and Animal Sciences, Jhang 35200, Sub-Campus University of Veterinary and Animal Sciences, Lahore 54000, Pakistan ]
Min Jung Kim [ Department of Theriogenology and Biotechnologies, College of Veterinary Medicine, Seoul National University ]
Corresponding Author