Earticle

초록

영어

This study used adult wistar-based rats to observe the sexual cycle as a morphological characteristic of vaginal epithelial cells by vaginal smearing, and investigated the fetal number through mating with male rats of the same strain. The target animal was a 12 to 13-week-old Wistar-based mature unlighted rat (weight 220 g to 240 g), room temperature 23 ± 2℃, 14 hours artificial lighting (05:00 to 19:00 hours), 10 hours Adapted individuals were used for rearing for at least 2 weeks under the conditions of the darkroom (19:00 to 05:00). The feed was managed for free feeding of pellet feed for animals and water. The vaginal smearing method was used for the experiments by observing the sexual cycle every morning and confirming that the normal sexual cycle of 4 or 5 days was repeated at least 2 cycles or more. As a result, the proestrus was found to have few red blood cells, the cells and nuclei were rather large and round, and many nucleated cells were identified. In the case of the estrus, the cells were large and the nuclei were not stained, and most of the keratinocytes were found. In addition, in the metestrus and diestrus, there were many white blood cells, and it was confirmed that nucleated epithelial cells and keratinocytes were significantly reduced. The pregnancy period was 21 ± 1.8 days, and the number of live births per delivery was 11.9 on average. The number of fetuses on the 8th and 10th days of pregnancy were 15.2 ± 0.4 and 15.4 ± 0.3, respectively. On the contrary, the number of fetuses on the 12th day of pregnancy was 12.9 ± 0.6, which was significantly (p < 0.05) decreased compared to the 10th day of pregnancy, and the number of fetuses was similar until delivery. As a result of investigating the change of body weight according to the birth weight and growth stage after delivery, the birth weight of female and male was 9.2 ± 2.0 g and 9.8 ± 2.5 g, respectively. After that, until the 16th day, the female and the male showed similarly moderate weight gain, and then showed a rapid weight gain until the 21st day of lactation. With reference to the results of this study, it is expected to be used as basic data for determining the mating time of rodents and controlling pregnancy and fetal number.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
General information
Observation of estrus cycle by vaginal smearing
Changes in the number of fetuses during pregnancy
Statistics processing
RESULTS
Confirmation of estrus cycle by morphological characteristics of vaginal mucus cells
Changes in the number of fetuses during pregnancy
Changes in male and female weight after delivery
DISCUSSION
CONFLICTS OF INTEREST
AUTHOR CONTRIBUTIONS
AUTHOR’S POSITION AND ORCID NO.
REFERENCES

키워드

저자

• Seung-Hee Choi [ Department of Companion Animal, Seoul Hoseo Occupational Training College, Animal Biotechnology, Graduate School of Animal Life Convergence Science, Institute of Genetic Engineering, Hankyong National University ]
• Yong-Seong Cho [ Department of Companion Animal, Seoul Hoseo Occupational Training College ]
• Min-Ji Kim [ Department of Companion Animal, Seoul Hoseo Occupational Training College ]
• Chae-Hyeok Lee [ Department of Companion Animal, Seoul Hoseo Occupational Training College ]
• Hwan-Hoo Seong [ Department of Companion Animal, Seoul Hoseo Occupational Training College ]
• Soon-Hwa Baek [ Department of ICT, Baekseok University ]
• Jang-Hee Lee [ Department of Companion Animal, Seoul Hoseo Occupational Training College ] Corresponding Author

참고문헌

발행기관

간행물

관심저널 등록

• 간행물명

  Journal of Animal Reproduction and Biotechnology

• 간기

  계간

• pISSN

  2671-4639

• eISSN

  2671-4663

• 수록기간

  2019~2026

• 십진분류

  KDC 527 DDC 636