Earticle

초록

영어

Karyotype analysis is a major work in the process of triploid abalone production for the purpose of productivity and quality improvement. However, the metaphase spreads for karyotype analysis have been prepared just from the larvae at trochophore stage, which has restricted the spectrum of sample correction inhibiting more efficient analysis. Here, we investigated the feasibility of preparing metaphase spreads from the larvae at veliger stage that is the next developmental stage of trochophore. For this, diploid and triploid larvae at trochophore and veliger stages from Pacific abalone (Haliotis discus hannai ) were subjected to metaphase spread preparation and its efficiencies were measured and compared each other. As the results, although the efficiencies of metaphase spread preparation were significantly lower in the larvae at veliger stage compared to the ones at trochophore stage regardless of ploidy status, we found that the preparation of metaphase spreads, which showed the clear chromosomal images containing the normal number of chromosomes, was possible from the veliger stage larvae. On the other hands, all larvae used in this study regardless of developmental stage and ploidy did not show colchicine sensitivity. Moreover, no significant difference was observed in cell cycle distribution of the cells comprising larvae between two developmental stages regardless of ploidy status. These suggested that the details of protocol to prepare metaphase spreads from abalone larvae should be optimized depending on its developmental stages. Taken together, we demonstrated the feasibility of preparing metaphase spreads from H. discus hannai veliger stage larvae for karyotype analysis.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Artificial fertilization and triploid induction
Metaphase spread preparation
Measurement of colchicine sensitivity
Analysis of cell cycles
Statistical analysis
RESULTS
Efficiency of metaphase spread preparation according to developmental stages of larvae
Normality of metaphase spreads in regard to the number of chromosome sets
Colchicine sensitivity
Cell cycle distribution of the cells comprising the larvae
DISCUSSION
CONCLUSIONS
CONFLICTS OF INTEREST
ACKNOWLEDGEMENTS
AUTHOR CONTRIBUTIONS
AUTHOR’S POSITION AND ORCID NO.
REFERENCES

키워드

저자

• Jae Hoon Choi [ Department of Fisheries Biology, Pukyong National University ]
• Eun Jeong Kim [ Department of Fisheries Biology, Pukyong National University ]
• Choul-Ji Park [ Genetics and Breeding Research Center, National Institute of Fisheries Science ]
• Yoon Kwon Nam [ Genetics and Breeding Research Center, National Institute of Fisheries Science, Department of Marine-Biomaterials and Aquaculture, Pukyong National University, Busan 48513, Korea ]
• Seung Pyo Gong [ Genetics and Breeding Research Center, National Institute of Fisheries Science, Department of Marine-Biomaterials and Aquaculture, Pukyong National University, Busan 48513, Korea ] Corresponding Author

참고문헌

발행기관

간행물

관심저널 등록

• 간행물명

  Journal of Animal Reproduction and Biotechnology

• 간기

  계간

• pISSN

  2671-4639

• eISSN

  2671-4663

• 수록기간

  2019~2026

• 십진분류

  KDC 527 DDC 636