Earticle

초록

영어

Ashwagandha is an important ancient medicinal crops, being affected with many diseases, among which leaf blight caused by Alternaria alternata has become the constraint resulting in huge yield losses. Continuous usage of chemical methods leads to environment, soil and water pollution. Whereas biological control of diseases is long lasting, inexpensive, eco-friendly and harmless to target organisms. In this context, it is aimed to evaluate five Trichoderma strains viz. Trichoderma virens IMI-392430, T. pseudokoningii IMI-392431, T. harzianum IMI-392432, T. harzianum IMI-392433 and T. harzianum IMI-392434 as bio-control efficacy against A. alternata and growth promoting effect in Ashwagandha. All the Trichoderma strains had varied antagonistic effects against the pathogen. In dual culture technique, the strain T. harzianum IMI-392433 showed maximum percentage inhibition of mycelial growth (54.89%) followed by T. harzianum IMI-392432 (53.83%), T. harzianum IMI-392434 (48.94%) and T. virens IMI-392430, (43.62%) against the pathogen, while the least inhibition percentage was observed with the T. pseudokoningii IMI-392431 (36.60%). The culture filtrate of the Trichoderma strain, T. harzianum IMI-392433 recorded highest inhibition on the mycelial growth (39.05%) and spore germination (80.77%) of pathogen and the lowest was recorded in T. pseudokoningii IMI-392431 (20.45 and 50%). Moreover, seeds treated with spore suspension of the strain T. harzianum IMI-392433 reduced the percentages of disease severity index significantly. The strain T. harzianum IMI-392433 also significantly increased seed germination %, seedling vigor and growth of Ashwagandha. The correlation matrix showed that root yield per plant of Ashwagandha had significant and positive correlation with plant height (r=0.726**), number of leaf (r=0.514**), number of primary branch (r=0.820**), number of secondary branch (r=0.829**), fresh plant weight (r=0.887**), plant dry weight (r=0.613**), root length (r=0.824**), root diameter (r=0.786**), root dry weight (r=0.739**) and fresh root weight (r=0.731**). The significant and negative correlation (r=-0.336**) was observed with the root yield and percentages of disease severity index. The study recognized that the T. harzianum IMI-392433 strain performed well in inhibiting the mycelial growth and reduced the percentages of disease severity index of pathogen as well as increased the plant growth in Ashwagandha.

목차

Abstracts
Introduction
Materials and Methods
Isolation and identification of pathogen
Morphology of the fungal pathogen
Pathogenicity test
Sources of Trichoderma strains
Antagonism of Trichoderma in dual culture on A. alternate
Effect of culture filtrates on conidial germination of pathogen
Effect of Trichoderma strains on growth of Ashwagandha in vivo
Preparation of pot
Preparation of inoculum
Seed treatment
Foliar spray
Data recording
Statistical analysis
Results and Discussion
Morphological and microscopic characteristics of A.alternata
Determination of inhibitory activity of culture fil-tratesof Trichoderma using normal poison agar technique
Effect of culture filtrates on conidial germination
Plant growth promotion
Conclusion
Acknowledgements
References

키워드

저자

• Md. Ahsanur Rahman [ Forest Protection Division, Bangladesh Forest Research Institute, Chattogram 40000, Bangladesh ] Corresponding Author
• Md. Arifur Rahman [ Seed Orchard Division, Bangladesh Forest Research Institute, Chattogram 4000, Bangladesh ]
• Zakiah Rahman Moni [ Technology Transfer and Monitoring Unit (TTMU), Bangladesh Agricultural Research Council (BARC), Farmget, Dhaka 1215, Bangladesh ]
• Mohammad Anisur Rahman [ Wood Preservation Division, Bangladesh Forest Research Institute, Chattogram 4000, Bangladesh ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Journal of Forest and Environmental Science [산림과학연구]

• 간기

  계간

• pISSN

  2288-9744

• eISSN

  2288-9752

• 수록기간

  1981~2026

• 등재여부

  KCI 등재

• 십진분류

  KDC 526 DDC 634