Earticle

초록

영어

Cryopreservation is used for blastocyst preservation of most mammalian embryos and is an important technique for breeding. We aimed to compare the efficiency of the cryopreservation method using the standard Cryotop device and the ReproCarrier device, a domestic product manufactured in Korea. The efficacy of the two devices was analyzed based on the survival rate, intracellular levels of reactive oxygen species (ROS), and apoptosis of the vitrified bovine blastocysts. The survival rates of the vitrified-warmed blastocysts were similar between the ReproCarrier group (58.4 ± 17.7%) and Cryotop group (59.9 ± 14.1%). Intracellular ROS levels and apoptotic index were determined by DCFDA staining and TUNEL assay. Changes in intracellular ROS levels, number of total nuclei, and cellular apoptosis of vitrified blastocysts after cryopreservation were not significantly different between the two groups. These results indicate that the ReproCarrier device method is as effective as the standard Cryotop method for vitrification of bovine blastocysts in vitro.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Chemicals
In vitro production of bovine embryos
Vitrification and warming procedure
Measurement of ROS levels
Assessment of apoptosis in blastocysts
Statistical analysis
RESULTS
Comparison of the instructions and features of the Cryotop and ReproCarrier products
Comparison of survival rate, intracellular ROS, and apoptosis level in the vitrified-warmed bovine blastocysts using Cryotop and ReproCarrier
DISCUSSION
CONCLUSION
CONFLICTS OF INTEREST
ACKNOWLEDGEMENTS
AUTHOR CONTRIBUTIONS
AUTHOR’S POSITION AND ORCID NO.
REFERENCES

키워드

저자

• Jin-Woo Kim [ Department of Biotechnology, College of Engineering, Daegu University, Institute of Infertility, Daegu University, Gyeongsan 38453, Korea ]
• Seul-Gi Yang [ Department of Biotechnology, College of Engineering, Daegu University, Institute of Infertility, Daegu University, Gyeongsan 38453, Korea ]
• Hyo-Jin Park [ Department of Biotechnology, College of Engineering, Daegu University, Institute of Infertility, Daegu University, Gyeongsan 38453, Korea ]
• Ju Hwan Kim [ Modu Science, Sejong 30128, Korea ]
• Dong-Mok Lee [ Biomedical Manufacturing Technology Center, Korea Institute of Industrial Technology, Yeongcheon 38822, Korea ]
• Seong-Min Woo [ Department of Biotechnology, College of Engineering, Daegu University ]
• Hyun-Jeong Kim [ Department of Biotechnology, College of Engineering, Daegu University ]
• Hyun Ah Kim [ Department of Biotechnology, College of Engineering, Daegu University ]
• Jae-Hoon Jeong [ Department of Biotechnology, College of Engineering, Daegu University ]
• Min Ji Lee [ Department of Biotechnology, College of Engineering, Daegu University ]
• Deog-Bon Koo [ Department of Biotechnology, College of Engineering, Daegu University, Institute of Infertility, Daegu University, Gyeongsan 38453, Korea ] Corresponding Author

참고문헌

발행기관

간행물

관심저널 등록

• 간행물명

  Journal of Animal Reproduction and Biotechnology

• 간기

  계간

• pISSN

  2671-4639

• eISSN

  2671-4663

• 수록기간

  2019~2025

• 십진분류

  KDC 527 DDC 636