Earticle

초록

영어

Partheno Embryo's research is known to play a very important role in identifying the development of embryonic cells or analyzing the genetic mechanisms of embryonic development, but the information on apoptosis formed during the early stage of development on Partheno Embryo is very little. Therefore, this study analyzed whether the embryonic cell death of unit embryos can be inhibited by adding Scriptaid, one of HDACi, which plays a role in demethylation of histone proteins as a method of regulating the cell cycle in the early embryo development of Partheno Embryo. As a result, the differentiation rate was higher in the group that added Scriptaid and FBS, but the cellular development was higher in the group that added pregnant serum to Scriptaid. As a result of analyzing the expression of the gene through IF and PCR, the group with the addition of gestational serum increased the expression of BCL2 and PCNA, which affects the anti-Casp3 action in cell survival. In addition, it is interpreted that treatment of Scriptaid for 16 hours, rather than 24 h treatment lowers the expression of Casp-3, a representative factor of apoptosis, and also increases embryonic development, thus affecting early embryo development. Therefore, it is concluded that the 16-hour treatment of Scriptaid and the use of gestational serum will inhibit cell death in the early embryonic development and increase the development rate of the embryo.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Chemicals and animal ethics statement
In vitro maturation
Parthenogenetic activation
In vitro culture
Enzyme linked immunosorbent assay
Zymography
Semi-quantitative polymerase chain reaction
Immunofluorescence
Statistical analyses
RESULTS
Changes in embryonic cells during in vitro culture
MMPs expression in embryonic cells
Analysis of gene expression patterns related to cell survival and apoptosis
DISCUSSION
CONFLICTS OF INTEREST
AUTHOR CONTRIBUTIONS
AUTHOR’S POSITION AND ORCID NO.
REFERENCES

키워드

저자

• Min-Gee Oh [ Department of Animal Life Science, Hankyong National University, Anseong 17579, Korea ]
• Na-Hyeon Jung [ Major in the Animal Biotechnology, Graduate School of Future Convergence Technology, Hankyong National University, Anseong 17579, Korea ]
• Dae-Seung Kim [ Major in the Animal Biotechnology, Graduate School of Future Convergence Technology, Hankyong National University, Anseong 17579, Korea ]
• Jong-Taek Yoon [ Department of Animal Life Science, Hankyong National University, Anseong 17579, Korea ] Corresponding Author

참고문헌

발행기관

간행물

관심저널 등록

• 간행물명

  Journal of Animal Reproduction and Biotechnology

• 간기

  계간

• pISSN

  2671-4639

• eISSN

  2671-4663

• 수록기간

  2019~2026

• 십진분류

  KDC 527 DDC 636