Earticle

초록

영어

Nitric oxide (NO)-induced protein S-nitrosylation triggers mitochondrial dysfunction and was related to cell senescence. However, the exact mechanism of these damages is not clear. In the present study, to investigate the relationship between in vitro aging and NO-induced protein S-nitrosylation, oocytes were treated with sodium nitroprusside dihydrate (SNP), and the resultant S-nitrosylated proteins were detected through biotin-switch assay. The results showed that levels of protein S-nitroso thiols (SNO)s and expression of S-nitrosoglutathione reductase (GSNOR) increased, while activity and function of mitochondria were impaired during oocyte aging. Addition of SNP, a NO donor, to the oocyte culture led to accelerated oocyte aging, increased mitochondrial dysfunction and damage, apoptosis, ATP deficiency, and enhanced ROS production. These results suggested that the increased NO signal during oocyte aging in vitro, accelerated oocyte degradation due to increased protein S-nitrosylation, and ROS-related redox signaling.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Collection and in vitro maturation of cattle oocytes
In vitro aging and SNP treatment
Biotin-switchd assay for detection of S-nitrosylated proteins
ROS measurements
Total and active mitochondrial staining
Immunofluorescence and confocal microscopy
Analysis of MtDNA copy number
ATP measurements
Statistical analysis
RESULTS
Protein SNO level and GSNOR expression increases during oocyte aging
Mitochondrial activity and function decrease during oocyte aging
Treatment with SNP increases protein SNO level and leads to accumulation of damaged mitochondria
Treatment with SNP increases ROS production and apoptosis level
DISCUSSION
CONFLICTS OF INTEREST
ACKNOWLEDGEMENTS
AUTHOR CONTRIBUTIONS
AUTHOR’S POSITION AND ORCID NO.
REFERENCES

키워드

저자

• Ying-Jie Niu [ Department of Animal Science, Chungbuk National University ]
• Dongjie Zhou [ Department of Animal Science, Chungbuk National University ]
• Wenjun Zhou [ Department of Animal Science, Chungbuk National University ]
• Zheng-Wen Nie [ Department of Animal Science, Chungbuk National University ]
• Ju-Yeon Kim [ Department of Animal Science, Chungbuk National University ]
• YoungJin Oh [ Chung Cheong Buk-Do Institute of Livestock and Veterinary Research, Cheongju 28153, Korea ]
• So-Rim Lee [ Chung Cheong Buk-Do Institute of Livestock and Veterinary Research, Cheongju 28153, Korea ]
• Xiang-Shun Cui [ Department of Animal Science, Chungbuk National University ] Corresponding Author

참고문헌

발행기관

간행물

관심저널 등록

• 간행물명

  Journal of Animal Reproduction and Biotechnology

• 간기

  계간

• pISSN

  2671-4639

• eISSN

  2671-4663

• 수록기간

  2019~2026

• 십진분류

  KDC 527 DDC 636