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Original Article

Study on the In-vitro Culture Method for Normal Embryonic Cell Development of Porcine Parthenogenetic Embryos

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  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Journal of Animal Reproduction and Biotechnology 바로가기
  • 통권
    Volume. 35 No. 1 (2020.03)바로가기
  • 페이지
    pp.94-101
  • 저자
    Na-Hyeon Jung, Sang-Hwan Kim, Dae-Seung Kim, Jong-Taek Yoon
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A378894

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원문정보

초록

영어
In the early development of parthenogenetic embryo, cytoplasm and nucleic acid fragmentation may be a cause of lower embryo development. The purpose of this study was to evaluate whether embryonic development and apoptosis factors can be reduced by controlling the in-vitro culture environment by the addition of hormones, pregnancy serum and uterine milk. Our study showed that the activity of Casp-3 increased within the cytoplasm when artificially used hormones to induce the incubation environment, and PCNA's manifestation was low. However, the addition of pregnant serum appeared to lower the Casp-3 activity compared to the other groups. In addition, MMP-9 activity was increased and early embryo development and cytoplasmic fidelity were also increased. Therefore, the results of the present study showed that the use of gestational serum in the development of parthenogenetic embryo inhibit apoptosis and increases cytoplasmic reorganization by natural environmental control in in vitro culture.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Chemicals and animal ethics statement
In vitro maturation
Parthenogenetic activation
In vitro culture
Enzyme linked immunosorbent assay
Zymography
Immunofluorescence
RESULTS
Differences of embryonic cell development in each in vitro culture method
Activity of MMPs according to embryonic cell development
Analysis of expression and localization of genes related to cell division and apoptosis.
DISCUSSION
CONFLICTS OF INTEREST
AUTHOR CONTRIBUTIONS
AUTHOR’S POSITION AND ORCID NO.
REFERENCES

저자

  • Na-Hyeon Jung [ Major in the Animal Biotechnology, Graduate School of Future Convergence Technology, Hankyong National University, Anseong 17579, Korea ]
  • Sang-Hwan Kim [ Institute of Genetic Engineering, Hankyong National University, Anseong 17579, Korea ]
  • Dae-Seung Kim [ Major in the Animal Biotechnology, Graduate School of Future Convergence Technology, Hankyong National University, Anseong 17579, Korea ]
  • Jong-Taek Yoon [ Institute of Genetic Engineering, Hankyong National University, Department of Animal Life Science, Hankyong National University, Anseong 17579, Kore ] Corresponding Author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Journal of Animal Reproduction and Biotechnology
  • 간기
    계간
  • pISSN
    2671-4639
  • eISSN
    2671-4663
  • 수록기간
    2019~2026
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / Journal of Animal Reproduction and Biotechnology Volume. 35 No. 1

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