Earticle

초록

영어

The establishment of porcine embryonic stem cells (ESCs) from porcine somatic cell nuclear transfer (SCNT) blastocysts is influenced by in vitro culture day of porcine reconstructed embryo and feeder cell type. Therefore, the objective of the present study was to determine the optimal in vitro culture period for reconstructed porcine SCNT embryos and mouse embryonic fibroblast (MEF) feeder cell type for enhancing colony formation efficiency from the inner cell mass (ICM) of porcine SCNT blastocysts and their outgrowth. As the results, porcine SCNT blastocysts produced through in vitro culture of the reconstructed embryos for 8 days showed significantly increased efficiency in the formation of colonies, compared to those for 7 days. Moreover, MEF feeder cells derived from outbred ICR mice showed numerically the highest efficiency of colony formation in blastocysts produced through in vitro culture of porcine SCNT embryos for 8 days and porcine ESCs with typical ESC morphology were maintained more successfully over Passage 2 on outbred ICR mice-derived MEF feeder cells than on MEF feeder cells derived from inbred C57BL/6 and hybrid B6CBAF1 mice. Overall, the harmonization of porcine SCNT blastocysts produced through in vitro culture of the reconstructed embryos for 8 days and MEF feeder cells derived from outbred ICR mice will greatly contribute to the successful establishment of ESCs derived from porcine SCNT blastocysts.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Animals
Retrieval of cumulus-oocyte complexes (COCs) and in vitro maturation (IVM)
Preparation of donor cells
Generation of somatic cell nuclear transfer (SCNT) embryos
In vitro culture of SCNT embryos
Preparation of MEF feeder cells
In vitro outgrowth of ICM of SCNT blastocysts
Statistical analysis
RESULTS
DISCUSSION
CONFLICTS OF INTEREST
ACKNOWLEDGEMENTS
AUTHOR CONTRIBUTIONS
AUTHOR’S POSITION AND ORCID NO.
REFERENCES

키워드

저자

• Na Rae Han [ Department of Animal Life Science, Kangwon National University, Chuncheon 24341, Korea ]
• Song Baek [ Department of Animal Life Science, Kangwon National University, Chuncheon 24341, Korea ]
• Yongjin Lee [ College of Veterinary Medicine, Kangwon National University, Optipharm Inc. ]
• Joohyeong Lee [ Institute of Veterinary Medicine, Kangwon National University, Chuncheon 24341, Korea ]
• Jung Im Yun [ KustoGen Inc., Chuncheon 24341, Korea ]
• Eunsong Lee [ College of Veterinary Medicine, Kangwon National University, Chuncheon 24341, Korea ]
• Seung Tae Lee [ Department of Animal Life Science, Kangwon National University, KustoGen Inc., Department of Applied Animal Science, Kangwon National University, Chuncheon 24341, Korea ] Corresponding Author

참고문헌

발행기관

간행물

관심저널 등록

• 간행물명

  Journal of Animal Reproduction and Biotechnology

• 간기

  계간

• pISSN

  2671-4639

• eISSN

  2671-4663

• 수록기간

  2019~2026

• 십진분류

  KDC 527 DDC 636