Earticle

초록

영어

This study was conducted to examine the oocyte recovery efficiency through having an OPU session once and twice a week. Also, the oocyte recovery efficiency was examined by using OPU after two and three months of rest period. Six cows were used for oocytes collection and were randomly divided into two groups. In experiment 1, OPU sessions were conducted once and twice a week to collect oocytes. The collected oocytes between once and twice OPU groups were classified into four groups (grade 1, 2, 3 and 4) according to the quality of cumulus cells and ooplasm. Based on the result, the percentage of collected oocytes per aspirated follicle number was similar between once and twice OPU session groups (65.5 ± 1.9 and 68.7 ± 1.4 vs.). However, the percentage of grade 1 oocytes from the twice OPU session group was significantly high compared with that of the once a week OPU session group (25.3 ± 0.9 and 32.5 ± 1.2% vs. once and twice session group, respectively, p < 0.05). In experiment 2, the group with three months of rest period tended to have a high percentage of collected oocyte compared with the group with two months of rest period (64.6 and 70.9% vs. 2 and 3 months rest group, respectively, p = 0.62). The percentage of grade 4 in the group with three months of rest period was significantly low compared with the group with two months of rest period group (27.3 and 36.5% vs. two and three months rest group, respectively, p = 0.05). In conclusion, twice a week OPU session is suitable for collection of high quality oocytes by using OPU, and three months of rest period is needed for the recovery of oocyte quality of a donor cow.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Selection of cows for experiment and feed management
Collection of COCs from donor Hanwoo cows and classification of COCs grade
Experimental design
Statistical analysis
RESULTS AND DISCUSSION
CONFLICTS OF INTEREST
ACKNOWLEDGEMENTS
AUTHOR'S AFFILIATION, POSITION AND ORCID NO.
REFERENCES

키워드

저자

• Sung-Sik Kang [ Hanwoo Research Institute, NIAS, RDA, Pyeongchang 25340, Korea ]
• Ui-Hyung Kim [ Hanwoo Research Institute, NIAS, RDA, Pyeongchang 25340, Korea ]
• Seok-Dong Lee [ Hanwoo Research Institute, NIAS, RDA, Pyeongchang 25340, Korea ]
• Myung-Suk Lee [ Hanwoo Research Institute, NIAS, RDA, Pyeongchang 25340, Korea ]
• Man-Hye Han [ Hanwoo Research Institute, NIAS, RDA, Pyeongchang 25340, Korea ]
• Sang-Rae Cho [ Hanwoo Research Institute, NIAS, RDA, Pyeongchang 25340, Korea ] Corresponding Author

참고문헌

발행기관

간행물

관심저널 등록

• 간행물명

  Journal of Animal Reproduction and Biotechnology

• 간기

  계간

• pISSN

  2671-4639

• eISSN

  2671-4663

• 수록기간

  2019~2025

• 십진분류

  KDC 527 DDC 636