Earticle

초록

영어

Interferon tau (IFNT) regulation, an anti-luteolytic factor produced by conceptuses of the ruminant ungulates, is essential for the maintenance of early pregnancy, but a definitive mechanism for its temporal transcription has not been elucidated. We and others have observed the T-box protein eomesodermin (EOMES) exhibited high mRNA expression in the ovine embryonic trophectoderm; thus, both caudal-relatedhomeobox-2 (CDX2) and EOMES coexist during the early stages of conceptus development. Objective of this study was to examine the effect of EOMES on ovine IFNT gene transcription when evaluated with CDX2, ETS2 and AP1 transcription factors implicated in the control of cell differentiation in the trophectoderm. In this study, quantitatively via reverse transcription-polymerase chain reaction (RT-PCR) analysis between ovine trophoblast cells was initially performed, finding that transcription factors CDX2 and ‘EOMES transcription factor mRNAs’ were specific to trophectoderm cells. These mRNAs were also found in days 15, 17, and 21 ovine conceptuses. Furthermore, human choriocarcinoma JEG3 cells (trophoblast cell line) were cotransfected with an ovine IFNT (-654bp)-luciferase reporter (-654-oIFNT-Luc) construct and several transcription factor expression plasmids. Cotransfection of the reporter construct with CDX2, ETS2 and AP1 increased transcription of -654-oIFNT-Luc by about 11-fold compared with transfection of the construct alone. When cells were initially transfected with EOMES followed by transfection with CDX2, ETS2 and/or AP1, the expression of -654-oIFNT-Luc was decreased. Also, EOMES factor inhibited the stimulatory activity of CDX2 alone. These results suggest that when conceptuses attach to the uterine epithelium, ovine IFNT gene transcription is down-regulated by an increase of EOMES factor expression in the attached ovine trophoblast cells.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Animals and RNA extraction and RT-PCR
Plasmid constructions
Cell culture and transient transfection
Nuclear protein extraction, Western blot analyses
Statistical analysis
RESULTS
Co-existence of CDX2 and EOMES mRNA, and proteins from expression constructs
Effects of EOMES on IFNT gene transcription in JEG3 cells
Effects of AP1, CDX2, ETS2 and EOMES on IFNT gene transcription in JEG3 cells
Examination of AP1, CDX2 and/or ETS2 binding sites on IFNT gene transcription
DISCUSSION
CONFLICTS OF INTEREST
ACKNOWLEDGEMENTS
AUTHOR’S AFFILIATION, POSITION AND ORCID NO.
REFERENCES

키워드

저자

• Min-Su Kim [ Dairy Science Division, National Institute of Animal Science, RDA, Cheonan 31000, Korea ]
• Hyun-Joo Lim [ Dairy Science Division, National Institute of Animal Science, RDA, Cheonan 31000, Korea ]
• Ji Hwan Lee [ Dairy Science Division, National Institute of Animal Science, RDA, Cheonan 31000, Korea ]
• Tae Young Hur [ Dairy Science Division, National Institute of Animal Science, RDA, Cheonan 31000, Korea ]
• Jun Kyu Son [ Dairy Science Division, National Institute of Animal Science, RDA, Cheonan 31000, Korea ] Corresponding Author

참고문헌

발행기관

간행물

관심저널 등록

• 간행물명

  Journal of Animal Reproduction and Biotechnology

• 간기

  계간

• pISSN

  2671-4639

• eISSN

  2671-4663

• 수록기간

  2019~2026

• 십진분류

  KDC 527 DDC 636