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Original Article

Seminal Attributes and Semen Cryo-banking of Nepalese Indigenous Achhami (Bos indicus) Bull under Ex-situ Conservation

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  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Journal of Animal Reproduction and Biotechnology 바로가기
  • 통권
    Volume. 34 No. 4 (2019.12)바로가기
  • 페이지
    pp.272-279
  • 저자
    Pankaj Kumar Jha, Saroj Sapkota, Neena Amatya Gorkhali, Bhoj Raj Pokharel, Ajeet Kumar Jha, Shishir Bhandari, Bhola Shankar Shrestha
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A378875

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초록

영어
The study was conducted to evaluate the seminal attributes and cryo-banking of Achhami (Bos indicus) bull semen. Of two Achhami bulls, 8 ejaculates from each bull were evaluated for seminal attributes. For semen freezing and cryo-banking, 4 ejaculates (having ≥2 mL semen volume, ≥75% of sperm motility and ≥1,000 × 106 cells/mL of sperm concentration) from each bull were used. Semen samples were diluted in egg-yolk-tris-citrate extender using a two-step dilution protocol, and were frozen in liquid nitrogen (LN2) vapour in a styrofoam box. The mean semen volume, colour, sperm mass activity, motility, viability, concentration, abnormal acrosome, midpiece and tail and, abnormal head of two Achhami bulls were 4.4 ± 0.5 mL vs. 2.5 ± 0.2 mL, 2.5 ± 0.1 vs. 2.4 ± 0.1, 3.5 ± 0.1 vs. 3.5 ± 0.1, 77.0 ± 1.1% vs. 78.3 ± 1.3%, 94.4 ± 0.5% vs. 91.0 ± 0.6%, 1137.7 ± 73.7 × 106 cells/mL vs. 1060.0 ± 44.3 × 106 cells/mL, 10.2 ± 0.5% vs. 10.3 ± 0.5% and 6.7 ± 0.5% vs. 8.2 ± 0.3%, respectively. The post-thawed sperm motility and viability were 53.0 ± 2.0% vs. 50.0 ± 0.0% and 80.2 ± 0.4% vs. 73.2 ± 0.7%, while evaluating by computer-assisted sperm analysis (CASA) system, the percentage of the progressive motility, fast motility, slow motility, local motility and immotile sperm were 75%, 68%, 7.4%, 16.6% and 8.6%, respectively. A total number of 620 doses semen straw were cryo-banked. Due to the acceptable post-thawed sperm motility and viability recorded, cryopreservation of Achhami semen is hereby recommended so as to preserve the Achhami breed. For further validation, the fertility will be observed from the produced frozen semen.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Animal and management
Semen collection and evaluation
Semen dilution and cooling
Freezing and thawing of spermatozoa
Statistical analysis
RESULTS
Seminal attributes of fresh semen
Post-thawed semen quality
Cryo-banking
DISCUSSION
CONCLUSION
CONFLICTS OF INTEREST
ACKNOWLEDGEMENTS
AUTHOR’S AFFILIATION, POSITION AND ORCID NO.
REFERENCES

키워드

Achhami bull Nepal semen cryopreservation

저자

  • Pankaj Kumar Jha [ Animal Breeding Division, Nepal Agricultural Research Council (NARC), Kathmandu 1950, Nepal ] Corresponding Author
  • Saroj Sapkota [ Animal Breeding Division, Nepal Agricultural Research Council (NARC), Kathmandu 1950, Nepal ]
  • Neena Amatya Gorkhali [ Animal Breeding Division, Nepal Agricultural Research Council (NARC), Kathmandu 1950, Nepal ]
  • Bhoj Raj Pokharel [ Animal Breeding Division, Nepal Agricultural Research Council (NARC), Kathmandu 1950, Nepal ]
  • Ajeet Kumar Jha [ Department of Veterinary Gynaecology and Obstetrics, West Bengal University of Animal and Fisheries Sciences, Kolkata 037, India ]
  • Shishir Bhandari [ Animal Breeding Division, Nepal Agricultural Research Council (NARC), Kathmandu 1950, Nepal ]
  • Bhola Shankar Shrestha [ Heifer International, Hattiban, Lalitpur 6043, Nepal ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Journal of Animal Reproduction and Biotechnology
  • 간기
    계간
  • pISSN
    2671-4639
  • eISSN
    2671-4663
  • 수록기간
    2019~2025
  • 십진분류
    KDC 527 DDC 636

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