Earticle

초록

영어

This study aimed to investigate the function of the constitutively activating mutation D540G on eel FSHR activity by in vitro functional studies. Site-directed mutagenesis was carried out to generate the D-to-G mutation at position 540 of the pcDNA3-eel FSHR construct. Vectors expressing either wild type or mutant receptor were transfected into Chinese hamster ovary (CHO-K1) cells. The functional characteristics of both the wild type and mutant receptors were analyzed by a cAMP assay. cAMP accumulation was highly increased in cells transfected with the D540G mutant receptor in a dose-dependent manner. Of note, basal cAMP levels were remarkably increased (~13.1-fold) with expression of this mutant when compared to wild type receptor. These findings suggest that the D540G mutation in the eel FSHR may contribute to ovulation during eel sex maturation as well as play a pivotal role in inducing FSHR activity.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Materials
Site-directed mutagenesis and vector construction
Transient transfection and production of rec-eelFSH protein
cAMP assay
Data analysis
RESULTS
DISCUSSION
CONFLICTS OF INTEREST
ACKNOWLEDGEMENTS
AUTHOR'S AFFILIATION, POSITION AND ORCID NO.
REFERENCES

키워드

저자

• Jeong-Soo Kim [ Animal Biotechnology, Graduate School of Future Convergence Technology, Hankyong National University ]
• Munkhzaya Byambaragchaa [ Animal Biotechnology, Graduate School of Future Convergence Technology, Hankyong National University ]
• Kwan-Sik Min [ Animal Biotechnology, Graduate School of Future Convergence Technology, Hankyong National University, Department of Animal Life Science, Institute of Genetic Engineering, Hankyong National University ] Corresponding Author

참고문헌

발행기관

간행물

관심저널 등록

• 간행물명

  Journal of Animal Reproduction and Biotechnology

• 간기

  계간

• pISSN

  2671-4639

• eISSN

  2671-4663

• 수록기간

  2019~2025

• 십진분류

  KDC 527 DDC 636