Earticle

초록

영어

In general, cloned pigs have been produced using the somatic cell nuclear transfer (SCNT) technique with various types of somatic cells; however, the SCNT technique has disadvantages not only in its low efficiency but also in the development of abnormal clones. This study aimed to compare early embryonic development and quality of SCNT embryos with those of induced pluripotent stem cells (iPSCs) NT embryos (iPSC-NTs). Ear fibroblast cells were used as donor cells and iPSCs were generated from these cells by lentiviral transduction with human six factors (Oct4, Sox2, c-Myc, Nanog, Klf4 and Lin28). Blastocyst formation rate in iPSC-NT (23/258, 8.9%) was significantly lower than that in SCNT (46/175, 26.3%; p < 0.05). Total cell number in blastocysts was similar between two groups, but blastocysts in iPSC-NT had a lower number of apoptotic cells than in SCNT (2.0 ± 0.6 vs. 9.8 ± 2.9, p < 0.05). Quantitative PCR data showed that apoptosis-related genes (bax, caspase-3, and caspase-9) were highly expressed in SCNT than iPSC-NT (p < 0.05). Although an early development rate was low in iPSC-NT, the quality of cloned embryos from porcine iPSC was higher than that of embryos from somatic cells. Therefore, porcine iPSCs could be used as a preferable cell source to create a clone or transgenic animals by using the NT technique.

목차

ABSTRACT
서론
재료 및 방법
난자 및 공여세포의 준비
핵이식란의 생산
Apoptosis 분석(TUNEL assay)
Embryonic quality 분석
통계분석
결과
돼지 iPSC 배양
체세포 및 iPSC 핵이식
배반포에서 유전자 발현 양상
고찰
결론
CONFLICTS OF INTEREST
ACKNOWLEDGEMENTS
ORCID
REFERENCES

키워드

저자

• 권대진 [ Dae-Jin Kwon | 전북대학교 국제농업개발협력센터 ]
• 오재돈 [ Jae-Don Oh | 전북대학교 동물생명과학과 ]
• 박미령 [ Mi-Ryung Park | 농촌진흥청 국립축산과학원 ]
• 황인설 [ In-Sul Hwang | 농촌진흥청 국립축산과학원 ]
• 박응우 [ Eung Woo Park | 농촌진흥청 국립축산과학원 ]
• 황성수 [ Seongsoo Hwang | 농촌진흥청 국립축산과학원 ] Corresponding Author

참고문헌

발행기관

간행물

관심저널 등록

• 간행물명

  Journal of Animal Reproduction and Biotechnology

• 간기

  계간

• pISSN

  2671-4639

• eISSN

  2671-4663

• 수록기간

  2019~2025

• 십진분류

  KDC 527 DDC 636