Earticle

초록

영어

Sestrin-2 (SESN2) as a stress-metabolic protein is known for its anti-oxidative effects as a downstream factor of PERK pathways in mammalian cells. However, the expression patterns of SESN2 in conjunction with the UPR signaling against to ER stress on porcine oocyte maturation in vitro, have not been reported. Therefore, we confirmed the expression pattern of SESN2 protein, for which to examine the relationship between PERK signaling and SESN2 in porcine oocyte during IVM. We investigated the SESN2 expression patterns using Western blot analysis in denuded oocytes (DOs), cumulus cells (CCs), and cumulus-oocyte complexes (COCs) at 22 and 44 h of IVM. As expected, the SESN2 protein level significantly increased (p < 0.01) in porcine COCs during 44 h of IVM. We investigated the meiotic maturation after applying ER stress inhibitor in various concentration (50, 100 and 200 μM) of tauroursodeoxycholic acid (TUDCA). We confirmed significant increase (p < 0.05) of meiotic maturation rate in TUDCA 200 μM treated COCs for 44 h of IVM. Finally, we confirmed the protein level of SESN2 and meiotic maturation via regulating ER-stress by only tunicamycin (Tm), only TUDCA, and Tm + TUDCA treatment in porcine COCs. As a result, treatment of the TUDCA following Tm pre-treatment reduced SESN2 protein level in porcine COCs. In addition, SESN2 protein level significantly reduced in only TUDCA treated porcine COCs. Our results suggest that the SESN2 expression is related to the stress mediator response to ER stress through the PERK signaling pathways in porcine oocyte maturation.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Chemicals
In vitro maturation (IVM)
Protein extraction and Western blot analysis
Immunofluorescence (IF) staining
Assessment of meiotic maturation
Statistical analysis
RESULTS
Changes in SESN2 protein level in porcine DOs, CCs, and COCs during IVM progression
Effects of inducing ER stress by Tm during meiotic maturation of porcine oocyte and PERK signaling activation in porcine COCs
Effects of TUDCA treatment on meiotic maturation of porcine COCs
Investigation of SESN2 protein expression in porcine COCs with Tm and/or TUDCA treatment during IVM
DISCUSSION
CONFLICTS OF INTEREST
ACKNOWLEDGEMENTS
ORCID
REFERENCES

키워드

저자

• Hyo-Jin Park [ Department of Biotechnology, College of Engineering, Daegu University, Institute of Infertility, Daegu University ]
• In-Su Kim [ Department of Biotechnology, College of Engineering, Daegu University, Institute of Infertility, Daegu University ]
• Jin-Woo Kim [ Department of Biotechnology, College of Engineering, Daegu University, Institute of Infertility, Daegu University ]
• Seul-Gi Yang [ Department of Biotechnology, College of Engineering, Daegu University, Institute of Infertility, Daegu University ]
• Min-Ji Kim [ Department of Biotechnology, College of Engineering, Daegu University, Institute of Infertility, Daegu University ]
• Deog-Bon Koo [ Department of Biotechnology, College of Engineering, Daegu University, Institute of Infertility, Daegu University ] Corresponding Author

참고문헌

발행기관

간행물

관심저널 등록

• 간행물명

  Journal of Animal Reproduction and Biotechnology

• 간기

  계간

• pISSN

  2671-4639

• eISSN

  2671-4663

• 수록기간

  2019~2026

• 십진분류

  KDC 527 DDC 636