Earticle

초록

영어

The Transgenic livestock can be useful for the production of disease-resistant animals, pigs for xenotranplantation, animal bioreactor for therapeutic recombinant proteins and disease model animals. Previously, conventional methods without using artificial nuclease-dependent DNA cleavage system were used to produce such transgenic livestock, but their efficiency is known to be low. In the last decade, the development of artificial nucleases such as zinc-finger necleases (ZFNs), transcription activator-like effector nucleases (TALENs) and clustered regulatory interspaced short palindromic repeat (CRISPR)/Cas has led to more efficient production of knock-out and knock-in transgenic livestock. However, production of knock-in livestock is poor. In mouse, genetically modified mice are produced by co-injecting a pair of knock-in vector, which is a donor DNA, with a artificial nuclease in a pronuclear fertilized egg, but not in livestock. Gene targeting efficiency has been increased with the use of artificial nucleases, but the knock-in efficiency is still low in livestock. In many research now, somatic cell nuclear transfer (SCNT) methods used after selection of cell transfected with artificial nuclease for production of transgenic livestock. In particular, it is necessary to develop a system capable of producing transgenic livestock more efficiently by co-injection of artificial nuclease and knock-in vectors into fertilized eggs.

목차

ABSTRACT
서론
유전자 가위(Programmable Nuclease)와 유전자 적중
유전자 가위를 이용한 유전자 편집에 의한 가축 생산
결론과 향후방향(미래 예측)
CONFLICTS OF INTEREST
ACKNOWLEDGEMENTS
ORCID
REFERENCES

키워드

저자

• 박다솜 [ Da Som Park | 전남대학교 농업생명과학대학 동물자원학부 ]
• 김소섭 [ Soseob Kim | 광개토한우 ]
• 구덕본 [ Deog-Bon Koo | 대구대학교 공과대학 생명공학과 ]
• 강만종 [ Man-Jong Kang | 전남대학교 농업생명과학대학 동물자원학부 ] Corresponding Author

참고문헌

발행기관

간행물

관심저널 등록

• 간행물명

  Journal of Animal Reproduction and Biotechnology

• 간기

  계간

• pISSN

  2671-4639

• eISSN

  2671-4663

• 수록기간

  2019~2026

• 십진분류

  KDC 527 DDC 636