Earticle

초록

영어

Although somatic cell nuclear transfer (SCNT)-derived embryonic stem cells (ESCs) in pigs have great potential, their use is limited because the establishment efficiency of ESCs is extremely low. Accordingly, we tried to develop in-vitro culture system stimulating production of SCNT blastocysts with high performance in the colony formation and formation of colonies derived from SCNT blastocysts for enhancing production efficiency of porcine ESCs. For these, SCNT blastocysts produced in various types of embryo culture medium were cultured in different ESC culture medium and optimal culture medium was determined by comparing colony formation efficiency. As the results, ICM of porcine SCNT blastocysts produced through sequential culture of porcine SCNT embryos in the modified porcine zygote medium (PZM)-5 and the PZM-5F showed the best formation efficiency of colonies in α-MEM-based medium. In conclusion, appropriate combination of the embryo culture medium and ESC culture medium will greatly contribute to successful establishment of ESCs derived from SCNT embryos.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Animals
Oocytes collection and in-vitro maturation (IVM)
Preparation of donor cells
Generation of somatic cell nuclear transfer (SCNT) embryos
In-vitro culture (IVC) medium used for culturing SCNT embryos
Production of SCNT blastocysts
Preparation of MEF feeder cells
In-vitro outgrowth of inner cell mass (ICM) of SCNT blastocysts
Statistical analysis
RESULTS
DISCUSSION
CONFLICTS OF INTEREST
ACKNOWLEDGEMENTS
ORCID
REFERENCES

키워드

저자

• Na Rae Han [ Department of Animal Life Science, Kangwon National University, Chuncheon 24341, Korea ]
• Song Baek [ Department of Animal Life Science, Kangwon National University, Chuncheon 24341, Korea ]
• Yongjin Lee [ College of Veterinary Medicine, Kangwon National University, Chuncheon 24341, Korea ]
• Joohyeong Lee [ Institute of Veterinary Medicine, Kangwon National University, Chuncheon 24341, Korea ]
• Jung Im Yun [ Institute of Animal Resources, Kangwon National University, Chuncheon 24341, Korea ]
• Eunsong Lee [ College of Veterinary Medicine, Kangwon National University, Chuncheon 24341, Korea ]
• Seung Tae Lee [ Department of Animal Life Science, Kangwon National University, Department of Applied Animal Science, Kangwon National University, Chuncheon 24341, Korea ] Corresponding Author

참고문헌

발행기관

간행물

관심저널 등록

• 간행물명

  Journal of Animal Reproduction and Biotechnology

• 간기

  계간

• pISSN

  2671-4639

• eISSN

  2671-4663

• 수록기간

  2019~2026

• 십진분류

  KDC 527 DDC 636