Earticle

초록

영어

Many transcription factors are involved in directing the growth of porcine oocytes. The localization and expression level of a given transcription factor often differ at each stage of early embryonic growth, which spans from fertilization to the formation of the blastocyst. A hallmark of the blastocyst stage is the separation of the endodermal and mesodermal ectoderm. The embryo's medium and its effects are known to be crucial during early development compared to the other developmental stages, and thus require a lot of caution. Therefore, in many experiments, early development is divided into the quality of oocyte and cumulus cells and used in experiments. We thought that we were also heavily influenced by genetic reasons. Here, we examined the expression patterns of five key transcription factors (CDX2, OCT4, SOX2, NANOG, and E-CADHERIN) during porcine oocyte development whose expression patterns are controversial in the pig to the literature. Antibodies against these transcription factors were used to determine the expression and localization of them during the early development of pig embryos. These results indicate that the expressions of key transcription factors are generally similar in mouse and pig early developing embryos, but NANOG and SOX2 expression appears to show species-specific differences between pig and mouse developing embryos. This work helps us better understand how the expression patterns of transcription factors translate into developmental effects and processes, and how the expression and localization of different transcription factors can crucially impact oocyte growth and downstream developmental processes.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Materials
Oocyte collection and maturity
Parthenogenetic (PA)
Fixation and immunostaining
RESULTS
Expression of OCT4 and CDX2 in porcine oocytes and embryos
Expression of NANOG in porcine oocytes and embryos
Expression of SOX2 in porcine oocytes and embryos
Expression of E-CADHERIN in porcine oocytes and embryos
DISCUSSION
CONFLICTS OF INTEREST
ACKNOWLEDGEMENTS
ORCID
REFERENCES

키워드

저자

• So Yeon Kim [ Division of Animal and Dairy Sciences, Chungnam National University, Daejeon 34134, Korea ]
• Tao Lin [ Division of Animal and Dairy Sciences, Chungnam National University, Daejeon 34134, Korea ]
• Joo Bin Lee [ Division of Animal and Dairy Sciences, Chungnam National University, Daejeon 34134, Korea ]
• Jae Eun Lee [ Division of Animal and Dairy Sciences, Chungnam National University, Daejeon 34134, Korea ]
• Hyun Young Shin [ Division of Animal and Dairy Sciences, Chungnam National University, Daejeon 34134, Korea ]
• Dong Il Jin [ Division of Animal and Dairy Sciences, Chungnam National University, Daejeon 34134, Korea ] Corresponding Author

참고문헌

발행기관

간행물

관심저널 등록

• 간행물명

  Journal of Animal Reproduction and Biotechnology

• 간기

  계간

• pISSN

  2671-4639

• eISSN

  2671-4663

• 수록기간

  2019~2026

• 십진분류

  KDC 527 DDC 636