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Original Article

Effect of Extenders with TCG and DMSO on the Viability of Rabbit Sperm

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Journal of Animal Reproduction and Biotechnology 바로가기
  • 통권
    Volume. 34 No. 2 (2019.06)바로가기
  • 페이지
    pp.100-105
  • 저자
    Yeol Eo, Sang Hwan Kim, Seong-Gyu Bang, Min-Gee Oh, Chan-Hee Park, Jong Taek Yoon
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A378852

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원문정보

초록

영어
The purpose of this study was to evaluate the effect of addition of ethylene glycol, glycerol and sucrose to TCG (Tris, Citric Acid, Glucose, Egg Yolk) and DMSO Frozen. The extender containing Egg yolk concentration (10%, 20%) affects viability and acrosome morphology of rabbit sperm. Sperm viability was then assessed for the freezing extenders TCGD (Tris + Citricacid + Glucose + DMSO), TCGED (Tris + Citricacid + Glucose + Egg yolk + DMSO), TCGGD (Tris + Citricacid + Glucose + Glycerol + DMSO) and TCGSD Tris + Citricacid + Glucose + Sucrose + DMSO) during thawing at 38oC. for 20 seconds, respectively. TCG + 10% egg yolk (viability: 77.0 ± 0.8, NAI: 73.3 ± 0.9) was significantly (sperm viability and normal acrosome interaction (NAI)) higher than TCG + 20% egg yolk (70.7 ± 1.1, 70.0 ± 0.9) in the sperm normalcy analysis according to the yolk concentration. TCGGD (53.4 ± 0.1, 62.3 ± 0.4), TCGSD (61.3 ± 0.0, 67.1 ± 0.1) sperm viability and normal acrosome interaction (NAI) in frozen spermatozoa are TCGD (46.4 ± 2.8 and 56.3 ± 1. 4) and TCGED (23.0 ± 1.1 and 54.6 ± 1.4) extenders was thawed at 38oC for 20 seconds. According to the results from each frozen bulking agent, sperm membrane integrity by hypotonic swelling test (HOST) analysis in TCGGD (59.8 ± 0.7), TCGSD (59.3 ± 0.5) was significantly high compared to other experimental groups (p < 0.05). In conclusion, these results suggested that TCGGD and TCGSD extenders enhance survivability of rabbit sperm after frozen-thawing.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Semen collection
Preparation of extender
Low-temperature storage and examination of survival rate of sperm
Semen freezing and examination of survival rate of sperm
Survival rate and acrosomal normaliztion
Plasma membrane function test
Statistical analysis
RESULTS
Low temperature preservation of rabbit semen
Survival and vitality of rabbit sperm according to the egg yolk addition concentration
Ability rate of sperm according to frozen extender
DISCUSSION
CONFLICTS OF INTEREST
ORCID
REFERENCES

키워드

extender HOST rabbit sperm TCG

저자

  • Yeol Eo [ Major in the Animal Biotechnology, Graduate School of Future Convergence Technology, Hankyong National University, Anseong 17579, Korea ]
  • Sang Hwan Kim [ Institute of Genetic Engineering, Hankyong National University, Anseong 17579, Korea ]
  • Seong-Gyu Bang [ Department of Animal Life Science, Hankyong National University, Anseong 17579, Korea ]
  • Min-Gee Oh [ Department of Animal Life Science, Hankyong National University, Anseong 17579, Korea ]
  • Chan-Hee Park [ Department of Animal Life Science, Hankyong National University, Anseong 17579, Korea ]
  • Jong Taek Yoon [ Institute of Genetic Engineering, Hankyong National University, Anseong 17579, Korea, Department of Animal Life Science, Hankyong National University, Anseong 17579, Korea ] Corresponding Author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Journal of Animal Reproduction and Biotechnology
  • 간기
    계간
  • pISSN
    2671-4639
  • eISSN
    2671-4663
  • 수록기간
    2019~2026
  • 십진분류
    KDC 527 DDC 636

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