Earticle

초록

영어

The aim of this study was to investigate effects of hyaluronidase during IVM on oocyte maturation, oxidative stress status, expression of cumulus expansion-related (PTX, pentraxin; GJA1, gap junction protein alpha 1; PTGS2, prostaglandin-endoperoxide synthase 2) and fatty acid metabolism-related (FADS1, delta-6 desaturase; FADS2, delta-5 desaturase; PPARα, peroxisome proliferator-activated receptor-alpha) mRNA, and embryonic development of porcine oocytes. The cumulus-oocyte complexes (COCs) were incubated with 0.1 mg/mL hyaluronidase for 44 h. Cumulus expansion was measured at 22 h after maturation. At 44 h after maturation, nuclear maturation, intracellular glutathione (GSH) and reactive oxygen species (ROS) levels were measured. Gene expression in cumulus cells was analyzed using real time PCR. The cleavage rate and blastocyst formation were evaluated at Day 2 and 7 after insemination. In results, expansion of cumulus cells was suppressed by treatment of hyaluronidase at 22 h after maturation. Intracellular GSH level was reduced by hyaluronidase treatment (p < 0.05). On the other hand, hyaluronidase increased ROS levels in oocytes (p < 0.05). Only PTGS2 mRNA was enhanced in COCs by hyaluronidase (p < 0.05). Population of oocytes reached at metaphase II stage was higher in control group than hyaluronidase treated group (p < 0.05). Both of cleavage rate and blastocyst formation were higher in control group than hyaluronidase group (p < 0.05). Our present results showed that developmental competence of porcine oocytes could be reduce by hyaluronidase via inducing oxidative stress during maturation process and it might be associated with prostaglandin synthesis. Therefore, we suggest that suppression of cumulus expansion of COCs could induce oxidative stress and decrease nuclear maturation via reduction of GSH synthesis and it caused to decrease developmental competence of mammalian oocytes.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Oocytes collection and in vitro maturation (IVM)
Assessment of cumulus cell expansion
Measurement of intracellular GSH and ROS levels
Evaluation of nuclear maturation
Quantitative real time PCR
In vitro fertilization and culture
Statistical analysis
RESULTS
Effects of hyaluronidase on cumulus expansion and oxidative stress status
Changes of mRNA expression by hyaluronidase
Nuclear maturation and embryonic development
DISCUSSION
CONFLICTS OF INTEREST
ACKNOWLEDGEMENTS
ORCID
REFERENCES

키워드

저자

• Ye-Eun Jeon [ College of Animal Life Sciences, Kangwon National University, Chuncheon 24341, Korea ]
• Yong Hwangbo [ College of Animal Life Sciences, Kangwon National University, Chuncheon 24341, Korea ]
• Hee-Tae Cheong [ College of Veterinary Medicine, Kangwon National University, Chuncheon 24341, Korea ]
• Choon-Keun Park [ College of Animal Life Sciences, Kangwon National University, Chuncheon 24341, Korea ] Corresponding Author

참고문헌

발행기관

간행물

관심저널 등록

• 간행물명

  Journal of Animal Reproduction and Biotechnology

• 간기

  계간

• pISSN

  2671-4639

• eISSN

  2671-4663

• 수록기간

  2019~2026

• 십진분류

  KDC 527 DDC 636