Earticle

초록

영어

Soy isoflavones have been reported to possess many physiological activities such as antioxidant activity and inhibition of cancer cell proliferation. This study investigated the photoprotective effects of soybean extract in human fibroblast cell line and hairless mice model. Human fibroblast was treated with soybean extract before and after ultraviolet B (UVB; 290-302 nm) irradiation. In the soybean extract treated group, the cells showed better resistance to ultraviolet (UV) than control group. The amount of type I collagen recovered from the soybean treated group was higher than the vehicle group exposed to UV-induced damage. Moreover, increased expression of metalloproteinases-1 as a result of UV irradiation was suppressed by the soybean extract. Female mice were orally administered soybean extract and irradiated with UVB light for 8 weeks. The effects of the soybean extract on the skin appearance, collagen deposition and epidermal thickness in the UV-damaged mouse skin were analyzed using histopathological methods. In soybean extract treated group, the skin had a better morphology than that of the control group. Furthermore, the amount of type I collagen was increased and overexpression of MMP-1 was reduced in the soybean extract group compared to vehicle group. Additionally, up-regulation of pro-inflammatory cytokines induced by UV irradiation was suppressed by dietary soybean extract treatment. It appears that soybean extract had a photoprotective effect, including anti-aging and anti-inflammatory effect, from UV-induced damage in not only human fibroblast, but also hairless mice. We confirmed that these effects were possibly due to promotion of collagen synthesis and inhibition of MMP-1 expression.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Chemicals and reagents
Preparation of soybean extracts
Cell culture
Animals and treatments
Cell viability
UVB irradiation
Histological evaluation
Real-Time quantitative PCR
Enzyme-linked immunosorbent assay (ELISA) for measuring type 1 collagen synthesis
Enzyme-linked immunosorbent assay (ELISA) for measuring MMP1
Statistical analysis
RESULTS
Determination of optimal condition for extracting soybean isoflavone
Cell viability test for determination of efficacy does of soybean extract
Effect of soybean extract on type 1 collagen synthesis in cultured fibroblast cells
Soybean extract suppresses UVB-induced MMP-1 overexpression in cultured fibroblasts
Clinical observation and histopathological observations
Soybean prevents UVB-induced collagen degradation in hairless mice
Soy isoflavone extract suppresses the expression of COX-2 after UVB exposure
DISCUSSION
CONFLICTS OF INTEREST
ACKNOWLEDGEMENTS
REFERENCES

키워드

저자

• Young-Chang Cho [ College of Pharmacy, Chonnam National University, Gwangju 61186, Korea ]
• Jae-Bok Han [ Department of Radiological Science, Dongshin University, Naju 58245, Korea ]
• Sang-Ik Park [ College of Veterinary Medicine, Chonnam National University, Gwangju 61186, Korea ] Corresponding Author

참고문헌

발행기관

간행물

관심저널 등록

• 간행물명

  Journal of Animal Reproduction and Biotechnology

• 간기

  계간

• pISSN

  2671-4639

• eISSN

  2671-4663

• 수록기간

  2019~2025

• 십진분류

  KDC 527 DDC 636