Earticle

초록

영어

Because mesenchymal stem cells (MSCs) maintain distinct capacities with respect to self-renewal, differentiation ability and immunomodulatory function, they have been highly considered as the therapeutic agents for cell-based clinical application. Of particular, differentiation condition alters characteristics of MSCs, including cellular morphology, expression of gene/protein and cell surface molecule, immunological property and apoptosis. However, the previous results for differentiation-related apoptosis in MSCs have still remained controversial due to varied outcomes. Therefore, the present study aimed to disclose periodical alterations of pro- and anti-apoptosis in MSCs under differentiation inductions. The human dental pulp-derived MSCs (DP-MSCs) were differentiated into adipocytes and osteoblasts during early (1 week), middle (2 weeks) and late (3 weeks) stages, and were investigated on their apoptosis-related changes by Annexin V assay, qRT-PCR and western blotting. The ratio of apoptotic cell population was significantly (p < 0.05) elevated during the early to middle stages of differentiations but recovered up to the similar level of undifferentiated state at the late stage of differentiation. In the expression of mRNA and protein, whereas expressions of pro-apoptosis-related makers (BAX and BAK) were not altered in any kind and duration of differentiation inductions, anti-apoptosis marker (BCL2) was significantly (p < 0.05) elevated even at the early stage of differentiations. The recovery of apoptotic cell population at the late stage of differentiation is expected to be associated with the response by elevation of anti-apoptotic molecules. The present study may contribute on understanding for cellular mechanism in differentiation of MSCs and provide background data in clinical application of MSCs in the animal biotechnology to develop effective and safe therapeutic strategy.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Chemicals
Cell isolation and culture
MSCs-specific molecules expression
Differentiation of MSCs
Apoptosis analysis by Annexin V assay
Apoptosis analysis by quantitative RT-PCR (qRT-PCR)
Apoptosis analysis by western blotting
Statistical analysis
RESULTS
Characterization of DP-MSCs
Apoptosis analysis by Annexin V assay
Investigation for apoptosis analysis by quantitative RT-PCR (qRT-PCR) and western blotting
DISCUSSION
CONFLICTS OF INTEREST
ACKNOWLEDGEMENTS
REFERENCES

키워드

저자

• Hyeon-Jeong Lee [ College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea ]
• Byung-Joon Park [ College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Korea ]
• Ryoung-Hoon Jeon [ College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea ]
• Si-Jung Jang [ College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea ]
• Young-Bum Son [ College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea ]
• Sung-Lim Lee [ College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea ]
• Gyu-Jin Rho [ College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea ]
• Seung-Joon Kim [ College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Korea ]
• Won-Jae Lee [ College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Korea ] Corresponding Author

참고문헌

발행기관

간행물

관심저널 등록

• 간행물명

  Journal of Animal Reproduction and Biotechnology

• 간기

  계간

• pISSN

  2671-4639

• eISSN

  2671-4663

• 수록기간

  2019~2025

• 십진분류

  KDC 527 DDC 636