Earticle

초록

영어

We wanted to find the intensity of exercise that could increase brainderived neurotrophic factor (BDNF) expression and improve spatial learning and memory without dietary control. C57BL/6 mice were fed a 60% high-fat diet (HFD) for 6 weeks to induce obesity. Obesity-induced mice were exercised on a treadmill for 8 weeks at various exercise intensities: HFD-control (n= 7), HFD-low-intensity exercise (HFD-LIE, n= 7, 12 m/min for 75 min), HFD-middle intensity exercise (HFD-MIE, n= 7, 15 m/min for 60 min) and HFD-high-intensity exercise (HFD-HIE, n= 7, 18 m/min for 50 min). One week before sacrificing mice, the Morris water maze test was performed, and the hippocampus was immediately removed after sacrifice. The expression levels of BDNF (encoded by the gene Bdnf) and tropomyosin receptor kinase B (TrkB) in the hippocampus were analyzed by quantitative real-time reverse transcriptionpolymerase chain reaction and western blot. In the last probe test of the Morris water maze test, occupancy in the target quadrant was significantly higher in the HFD-HIE group (P< 0.05) than in the other groups. In addition, mRNA expression from the Bdnf promoter region was found to be significantly higher in the HFD-HIE group than in the other groups (P< 0.001). Although there were some differences in the levels of significance, the expression levels of both BDNF and TrkB were significantly higher in the HFD-HIE group than in the other groups. Therefore, relatively high-intensity aerobic exercise can resist the adverse effects of a high-fat diet on the brain without dietary control.

목차

Abstract
INTRODUCTION
MATERIALS AND METHODS
Animals
Exercise protocol
Morris water maze test
Quantitative real-time reverse transcription-polymerase chain reaction (qPCR)
Western blot
Statistical analysis
RESULTS
Relatively high-intensity exercise improved spatial learning and memory in obese mice despite sustained HFD
Relatively high-intensity exercise increased mRNA expression of hippocampal Bdnf in obese mice despite sustained HFD
Relatively high-intensity exercise increased hippocampal BDNF protein expression despite sustained HFD
DISCUSSION
CONFLICT OF INTEREST
REFERENCES

키워드

저자

• Tae-Won Kim [ Division of Sport Science, Pusan National University, Busan, Korea ]
• Kyung-Wan Baek [ Division of Sport Science, Pusan National University, Busan, Korea, Department of Parasitology and Tropical Medicine, School of Medicine, Pusan National University, Yangsan, Korea ]
• Hak Sun Yu [ Department of Parasitology and Tropical Medicine, School of Medicine, Pusan National University, Yangsan, Korea ]
• Il-Gyu Ko [ Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea ]
• Lakkyong Hwang [ Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea ]
• Jung-Jun Park [ Division of Sport Science, Pusan National University, Busan, Korea ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  JER [Journal of Exercise Rehabilitation]

• 간기

  격월간

• pISSN

  2288-176X

• eISSN

  2288-1778

• 수록기간

  2013~2026

• 등재여부

  SCOPUS,KCI 등재

• 십진분류

  KDC 517 DDC 613