Earticle

초록

영어

Eight morel mushroom species were collected from Korea and other countries. The culture characteristics, genetic relationships, and beta-glucan content of the strains were analyzed. The mycelia of Morchella species exhibited optimal growth when cultured in dark at 25 °C in media with pH 7. The mycelia had a distinctive mycelial scent and characteristically changed color, being white initially, and then turning dark yellow to dark brown as it grew. The mycelia were classified into five types based on morphology. The isolates were identified as Morchella conica, two M. sextelata, M. importuna, M. esculenta, and three M. crassipes, based on ITS-rDNA sequences. PCR polymorphisms were variably produced within Morchella spp. using Universal Fungal Fingerprinting Primers (UFPF) and classified into four groups at the intra and inter species level. The strains, KMCC04971 and KMCC04407, showed the same banding pattern as M. conica and M. sextelata, respectively; however, these results were different from those of ITS analysis. Glucan content analysis by strain showed that the KMCC 04973 strain of M. importuna had the highest alpha- and beta-glucan content, at 16.4 g and 33.1 g per 100 g, respectively.

목차

ABSTRACT
서론
재료 및 방법
공시균주
균사체 배양
Genomic DNA 추출
rDNA ITS 염기서열 분석
유전적 유연관계분석
PCR 다형성 분석
베타글루칸 함량 분석
통계처리
결과 및 고찰
배양적 특성 분석
ITS 염기서열에 의한 수집균주 계통분류 분석
PCR다형성에 의한 계통간 유연관계 분석
베타글루칸 함량 분석
적요
감사의 글
REFERENCES

키워드

저자

• 민경진 [ Gyeong-Jin Min | 농촌진흥청 국립원예특작과학원 인삼특작부 버섯과 ]
• 박혜성 [ Hye-sung Park | 농촌진흥청 국립원예특작과학원 인삼특작부 버섯과 ]
• 이은지 [ Eun-ji Lee | 농촌진흥청 국립원예특작과학원 인삼특작부 버섯과 ]
• 이찬중 [ Chan-Jung Lee | 농촌진흥청 국립원예특작과학원 인삼특작부 버섯과 ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국버섯학회지 [Journal of MUSHROOMS]

• 간기

  계간

• pISSN

  1738-0294

• eISSN

  2288-8853

• 수록기간

  2003~2025

• 등재여부

  KCI 등재

• 십진분류

  KDC 525 DDC 635