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Research Article

Effect of light-emitting diodes on cordycepin production in submerged Cordyceps militaris cultures

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  • 발행기관
    한국버섯학회 바로가기
  • 간행물
    한국버섯학회지 KCI 등재 바로가기
  • 통권
    제18권 제1호 (2020.03)바로가기
  • 페이지
    pp.10-19
  • 저자
    Si-Young Ha, Ji-Young Jung, Jai-Hyun Park, Dong-Hwan Lee, Ji-Won Choi, Jae-Kyung Yang
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A372527

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원문정보

초록

영어
Light is an important factor for cordycepin production in Cordyceps militaris. We investigated the effects of different light-emitting diode (LED) conditions including various LED wavelengths and their combinations on cordycepin production in Cordyceps militaris cultivated in submerged culture. The results of our study showed that the combinations of LED wavelengths were more beneficial than single LED sources for cordycepin production. Among the three tested wavelength combinations, the greatest effects for cordycepin production were observed for the red:blue light combination at the wavelength ratio of 5:5 or 3:7. The optimal culture conditions were 19.2278 h/day of illumination time; 9.19497 g/50 mL of glucose content in the media; and 53.112 h of cultivation time. Our model predicted a maximum yield of 2860.01 μg/mL cordycepin. Finally, to verify the calculated maximum, we performed experiments in the culture media representing the obtained optimum combination and the cordycepin yield of 2412.5 μg/mL.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Reagents and standards
Mycelia preparation
Submerged culture
Different wavelengths of LEDs
Combination of different ratios of wavelength of LEDs
Illumination time
Glucose content in media
Determination of mycelia dry weight
Determination of cordycepin
Experimental design by RSM
Statistical analysis
RESULTS AND DISCUSSION
Effect of various media on mycelia dry weight and production of cordycepin 
Effect of LEDs on mycelia dry weight and cordycepin content
Effects of LEDs combination on the mycelia dry weight and cordycepin content
Optimization of cordycepin production by RSM(BBD)
ACKNOWLEDGEMENTS
REFERENCES

키워드

Cordyceps militaris Cordycepin Light-emitting diode Response surface methodology Mushroom composition rdycepin

저자

  • Si-Young Ha [ Division of Environmental Forest Science/Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, 52828, Republic of Korea ]
  • Ji-Young Jung [ Division of Environmental Forest Science/Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, 52828, Republic of Korea ]
  • Jai-Hyun Park [ Division of Environmental Forest Science/Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, 52828, Republic of Korea ]
  • Dong-Hwan Lee [ Division of Environmental Forest Science/Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, 52828, Republic of Korea ]
  • Ji-Won Choi [ Division of Environmental Forest Science/Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, 52828, Republic of Korea ]
  • Jae-Kyung Yang [ Division of Environmental Forest Science/Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, 52828, Republic of Korea ] Corresponding Author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국버섯학회 [The Korean Society of Mushroom Science]
  • 설립연도
    1997
  • 분야
    농수해양>농학
  • 소개
    우리나라의 버섯 연구와 관련 산업의 발전에 기여하고 버섯관련 연구자와 관련산업 종사자간의 학술정보 등의 교류를 목적으로 한다.

간행물

  • 간행물명
    한국버섯학회지 [Journal of MUSHROOMS]
  • 간기
    계간
  • pISSN
    1738-0294
  • eISSN
    2288-8853
  • 수록기간
    2003~2025
  • 등재여부
    KCI 등재
  • 십진분류
    KDC 525 DDC 635

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