Earticle

초록

영어

Whole genome sequence of Abeliophyllum distichum Nakai (Oleaceae) cultivar Ok Hwang 1 Ho, which is Korean endemic species, was recently sequenced to understand its characteristics. Acteoside is one of major useful compounds presenting various activities, and its several proposed biochemical pathways were reviewed and integrated to make precise biochemical pathway. Utilizing MetaPre-AITM which was developed for predicting secondary metabolites based on whole genome with the precise biochemical pathway of acteoside and the InfoBoss Pathway Database, we successfully rescued all enzymes involved in this pathway from the genome sequences, presenting that A. distichum cultivar Ok Hwang 1 Ho may produce acteoside. High-performance liquid chromatography result displayed that callus of A. distichum cultivar Ok Hwang 1 Ho contained acteoside as well as isoacteoside which may be derived from acteoside. Taken together, we successfully showed that MetaPre-AITM can predict secondary metabolite from plant whole genomes. In addition, this method will be efficient to predict secondary metabolites of many plant species because DNA can be analyzed more stability than chemical compounds.

한국어

최근에 한국 고유종인 미선나무 (Abeliophyllum distichum Nakai; Oleaceae) 품종 옥황1호의 유전체가 성공적으로 해독되었다. Acteoside는 다양한 활성을 가지는 물질이며, 여러개의 생화학합성과정이 제시되어왔고, 이들을 통합 검토하여 정 확한 생화학합성과정을 완성하였다. 유전체 데이터로부터 2차대사산물을 예측할 수 있는 MetaPre-AITM와 정확한 acteoside 생화학합성과정, InfoBoss Pathway Database를 활용하여, acteoside에 관여하는 모든 효소의 유전자를 옥황1호 유전체로부 터 성공적으로 확인하였다. 이는 옥황1호는 acteoside 물질을 생산할 수 있는 가능성이 있음을 의미한다. 이에 고성능액체크로 마토그래피를 사용하여 옥황1호의 캘러스 세포를 분석하여 acteoside과 이의 유도체인 isoacteoside를 확인하였다. 본 연구는 MetaPre-AITM은 유전체로부터 2차대사산물을 성공적으로 예측하였다. 이 방법은 화학물질보다 안정적인 DNA를 분석하여 2 차 대사산물을 예측하는 효율적인 방법이 될 것이다.

목차

Abstract
요약
1. Background
2. Materials & Methods
2.1 Preparation of callus of A. distichum cultivar Ok Hwang 1ho
2.2 Identification of enzymes related to biochemical pathways of acteoside
2.3 Protocol of high-performance liquid chromatography (HPLC) for identifying acteoside
3. Results & Discussions
3.1 Integrated biochemical pathways of acteoside based on proposed multiple acteosides biochemical pathways
3.2 Prediction of biochemical pathway of acteoside from whole genome sequences of A. distichum cultivar Ok Hwang 1ho
3.3 Prediction of biochemical pathway of acteoside from whole genome sequences of A. distichum cultivar Ok Hwang 1ho
4. Conclusions
REFERENCES

키워드

Acteoside 생화학 합성과정 유전체 MetaPre-AITM InfoBoss Pathway Database Abeliophyllum distichum 옥황1호 Acteoside biochemical pathway Whole genome MetaPre-AITM InfoBoss Pathway Database Abeliophyllum distichum cultivar Ok Hwang 1ho

저자

• Jaeho Park [ 박재호 | Professor, Department of Pharmaceutical Science, JungWon University ]
• Hong Xi [ 시홍 | Senior Researcher, InfoBoss Co., Ltd. & InfoBoss Research Center ]
• Jiyun Han [ 한지윤 | Researcher, InfoBoss Co., Ltd. & InfoBoss Research Center ]
• Jeongmin Lee [ 이정민 | Researcher, InfoBoss Co., Ltd. & InfoBoss Research Center ]
• Yongsung Kim [ 김용성 | Team Leader, InfoBoss Co., Ltd. & InfoBoss Research Center ]
• Jun-mi Lee [ 이준미 | Researcher, InfoBoss Co., Ltd. & InfoBoss Research Center ]
• Janghyuk Son [ 손장혁 | CEO, InfoBoss Co., Ltd. & InfoBoss Research Center ]
• Joungjwa Ahn [ 안정좌 | Professor, Department of Food Science & Technology, JungWon University ]
• Taewon Jang [ 장태원 | Student, Department of Medicinal Plant Resources, Andong National University ]
• Jisoo Choi [ 최지수 | Student, Department of Pharmaceutical Science, JungWon University ]
• Jongsun Park [ 박종선 | CEO, InfoBoss Co., Ltd. & InfoBoss Research Center ] Corresponding Author

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