ABSTRACT 1. Introduction 2. Materials and methods 2.1. Materials 2.2. Extraction of crude polysaccharide, fucoidan and active fractions 2.3. Chemical analysis and evaluation of heavy metal content 2.4. Analysis of the distribution of molecular weights of JHCF4 2.5. Fourier-transform infrared specσoscopy (FT-lR) characterization 2.6. Cell culture 2.7. Nuclear staining with Hoechst 33342 2.8. Apoptotic and necrotic body formation 2.9. Apoptosis analysis by flow cytometry 2.10. Determination of reactive oxygen species (ROS) production 2.11. Determination of mitochondrial membrane potential 2.12. Measurement of cytochrome c release 2.13. Western blot analysis 2.14. Statistical analysis 3. Results and discussion 3.1. Cytotoxicity and anticancer effects of CPs in vitro 3.2. Heavy metal ion content 3.3 Chemical composition of active fractions and agarose gel electrophoresis 3.4. FT-IR analysis of fucoidan 3.5. Cytotoxicity and anticancer effects of JHCF and active fractions in vitro 3.6. JHCF4 induced apoptotic morphology of Hep3B cells 3.7. lnduction of ROS generation, mitochondrial membrane potential, and cytochrome c release in JHCF4-induced apoptosis 3.8. Apoptosis pathway regulation by JHCF4 4. Conclusion Acknowledgements References
Yu-Lin Dai [ Department of Marine Life Sciences, jeju National University, jilin Ginseng Academy, Changchun University of Chinese Medidne, Changchun 130117, China ]
Yun-Fei Jiang [ Department of Marine Life Sciences, jeju National University ]
Hyo Geun Lee [ Department of Marine Life Sciences, jeju National University ]
You-Jin Jeon [ Department of Marine Life Sciences, jeju National University, Marine Science lnstitute, Jeju National University, Jeju Self-Governing Province 63333 , Republic of Korea ]
Corresponding author
Min-Cheol Kang [ Research group of Food Processing, Research Division of Strategic Food Technology, Korea Food Research Institute (KFRI), Wanju 55365, Republic of Korea ]
Corresponding author
제주대학교 해양과학연구소 [Marine Science Institute Jeju National University]
설립연도
1968
분야
농수해양>수산학
소개
해양의 실체와 그 자원의 합리적 이용과 관리방법을 규명하기 위하여 해양물리, 화학, 생물, 지질, 공학등 여러 관련분야에 관한 다양한 연구를 수행하며, 환경의 보전과 보호를 위해서 수질, 대기, 상·하수도, 폐기물, 사회적 환경 등의 연구를 통하여 쾌적하고 청정한 환경을 보전하기 위한 다양한 연구를 수행하고, 또한 해양 및 환경분야의 우수한 인력양성, 국내외 학술교류의 증진, 학·산 협동체제의 확립 등을 통하여 해양과 환경에 관한 기초 및 응용적 학술연구를 종합적으로 수행함을 목적으로 한다.
간행물
간행물명
해양과학연구소 연구논문집 [Bulletin of the Marine Science Institute]