Earticle

초록

영어

Mesenchymal stem cells (MSCs) are known to differentiate into multiple lineages, making neurogenic differentiation an important target in the clinical field. In the present study, we induced the neurogenic differentiation of cells using histone deacetylase (HDAC) inhibitors and studied their mechanisms for further differentiation in vitro. We treated cells with the HDAC inhibitors, MS-275 and NaB; and found that the cells had neuron-like features such as distinct bipolar or multipolar morphologies with branched processes. The mRNA expressions encoding for NEFL, MAP2, TUJ1, OLIG2, and SYT was significantly increased following HDAC inhibitors treatment compared to without HDAC inhibitors; high protein levels of MAP2 and Tuj1 were detected by immunofluorescence staining. We examined the mechanisms of differentiation and found that the Wnt signaling pathway and downstream mitogen-activate protein kinase were involved in neurogenic differentiation of MSCs. Importantly, Wnt4, Wnt5a/b, and Wnt11 protein levels were highly increased after treatment with NaB; signals were activated through the regulation of Dvl2 and Dvl3. Interestingly, NaB treatment increased the levels of JNK and upregulated JNK phosphorylation. After MS-275 treatment, Wnt protein levels were decreased and GSK-3β was phosphorylated. In this cell, HDAC inhibitors controlled the non-canonical Wnt expression by activating JNK phosphorylation and the canonical Wnt signaling by targeting GSK-3β.

목차

Abstract
1. Introduction
2. Methods
2.1. Cell lines and reagents
2.2. Immunocytochemistry
2.3. Reverse Transcriptase-polymerase Chain Reaction (RT-PCR) Analysis
2.4. Quantitative RT-PCR
2.5. Western Blot Assay
2.6. Statistics
3. Results
3.1. HDAC Inhibitors Enhance Neuron-like Cell Differentiation of hBM-MSCs in Vitro
3.2. HDAC Inhibitors Regulate Wnt Pathway and Downstream JNK Signaling
4. Discussion
5. Conclusions
Acknowledgments
References

키워드

저자

• Sujeong Jang [ Department of Physiology, Chonnam National University Medical School, Jellanamdo 58128, Korea ]
• Seokho Park [ Department of Physiology, Chonnam National University Medical School, Jellanamdo 58128, Korea ]
• Hyong-Ho Cho [ Department of Otolaryngology-Head and Neck Surgery, Chonnam National University Medical School, Gwangju 61469, Korea ]
• Ung Yang [ Department of Horticulture, Asian Pear Research Institute, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, Korea ]
• Maru Kang [ Department of Defense Science & Technology, Gwangju University, Gwangju 61743, Korea ]
• Jong-Seong Park [ Department of Physiology, Chonnam National University Medical School, Jellanamdo 58128, Korea ]
• Sah-Hoon Park [ Department of Physiology, Chonnam National University Medical School, Jellanamdo 58128, Korea ]
• Han-Seong Jeong [ Department of Physiology, Chonnam National University Medical School, Jellanamdo 58128, Korea ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  통합자연과학논문집(구 조선자연과학논문집) [Journal of Integrative Natural Science]

• 간기

  계간

• pISSN

  2005-1042

• 수록기간

  2008~2025

• 등재여부

  KCI 등재

• 십진분류

  KDC 405 DDC 505