Earticle

초록

영어

The radish skin and radish greens are an edible part of the radish. But they are removed before eating the radish and used as a byproduct or an animal feed material because of their tough and rough texture. Melanin is a pigment that gives colour to our skin. But increased production of melanin can turn into benign or malignant tumours. These days due to global warming, the amount of Ultra violet (UVB) rays has been extensively increased with sunlight. Due to this, a phenomenon called exogenous photo aging is widely observed for all skin colour and types. As a result of this phenomenon, a set of enzymes called matrix metalloproteinases (MMP’s) that serves as degradation enzymes for extracellular matrix proteins mainly collagen is increased, causing depletion in collagen and resulting in early wrinkles formation. Therefore in our study we used the murine melanoma cell line B16/F10 to study the melanogenesis inhibition by Heated radish extract (HRE) in vitro and we used HRM-2 hair less mice exposed to artificial UVB for checking the efficacy of Heated radish extract in vivo. Furthermore, we prepared a 3% Heated radish extract (HRE) cream and checked its effects on human skin. Our results have clearly demonstrated that Heated radish extract (HRE) have potently suppressed the tyrosinase activity and melanin production in B16/F10 cells. It had also reduced the expression of components involved in melanin production pathway both transcriptionally and transitionally. In in vivo studies, HRE had potently suppressed the expression of MMP’s and reduced the wrinkle formation and inhibited collagen degradation. Moreover, on human skin, ginseng cream increased the resilience, skin moisture and enhanced the skin tone. Therefore in light of these findings, we conclude that HRE is an excellent skin whitening and antiaging product.

목차

Abstract
1. INTRODUCTION
2. EXPERIMENT MATERIALS AND METHODS
2.1. Chemicals and Reagents
2.2. Preparation of Heat-Treated Radish Extract (HRE)
2.3. Cell Line
2.4. Cell-free Tyrosinase inhibition assay
2.5. Melanin inhibition assay
2.6. Animal experiment and grouping
2.7. UVB irradiation and induction of photo aging
2.8. Effect on melanin production in HRM-2 mice
2.9. Skin wrinkle measurement
2.10. Enzyme-linked immunosorbent assay (ELISA)
2.11. Histological observation of skin
2.12. RNA extraction and Real-Time PCR
2.13. Western blot analysis
2.14. Statistical analysis
3. RESULT AND DISCUSSION
3.1. Heated radish extract (HRE) inhibited tyrosinase activity in cell-free system and suppressed the melanin content
3.2. Effects of Heated radish extract on the mRNA and protein expressions of MITF and tyrosinase related proteins
3.3. Effect of oral supplementation of HRE on body weight and food intake
3.4. Analysis of wrinkle-related genes expression in UVB irradiated skin damage induction model
3.5. Effects of HRE on Melanogenesis in Skin Damage Induced by UVB Irradiation
3.6. Analysis
3.7. Decrease in epidermal thickness and increase in collagen by HRE
5. CONCLUSION
ACKNOWLEDGMENTS
REFERENCES

키워드

저자

• Hyun-Kyoung Kim [ Professor , Department of Food Science and Engineering, Seowon University, Cheongju, Korea ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  International Journal of Advanced Culture Technology(IJACT)

• 간기

  계간

• pISSN

  2288-7202

• eISSN

  2288-7318

• 수록기간

  2013~2025

• 등재여부

  KCI 등재

• 십진분류

  KDC 600 DDC 700