Component analysis of four-part extracts from Chamaecyparis obtusa Endl. by supercritical fluid extraction and anti-inflammatory effect on RAW 264.7cells
Byeong Mun Kwak, Ee-Hwa Kim, Yong-Min Kim, Hee-Tack Kim
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영어(ENG)
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https://www.earticle.net/Article/A363858
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The main purpose of this study was to suggest a solution for inflamma-tion caused by urban daily life based on supercritical extraction of Cha-maecyparis obtusa Endl. (C. obtusa). The leaves, cone, wood, and bark of C. obtusa were separately extracted with supercritical extraction. Al-pha-pinene, anti-inflammatory active compound of C. obtusa, was ana-lyzed by electrospray ionization-mass spectrometry, gas chromatogra-phy, and gas chromatography mass spectrometry from each part of ex-tracts. As a result of the reduction rate of nitric oxide in RAW 264.7 cells, the wood extract exhibited the highest reduction rate was about 45% among extracts from four part of C. obtusa. The wood extract from C. obtusa can be developed as a good anti-inflammatory natural raw ma-terial for natural product industry.
목차
Abstract INTRODUCTION MATERIALS AND METHODS Supercritical fluid extraction Fatty acid analysis Electrospray ionization-mass spectrometry Solid phase microextraction method Nitric oxide assay RESULTS Supercritical fluid extraction Fatty acid component GC/MS analysis Effects of extracts from C. obtusa and α-pinene on NO DISCUSSION CONFLICT OF INTEREST ACKNOWLEDGMENTS REFERENCES
Byeong Mun Kwak [ Department of Meridian & Acupoint, College of Korean Medicine, Semyung University, Jecheon, Korea ]
Ee-Hwa Kim [ Department of Meridian & Acupoint, College of Korean Medicine, Semyung University, Jecheon, Korea ]
Yong-Min Kim [ Department of Oriental Medical and Herbal Cosmetic Sciences, Semyung University, Jecheon, Korea ]
Hee-Tack Kim [ Department of Korean Medical Ophthalmology & Otolaryngology & Dermatology, College of Korean Medicine, Semyung University, Jecheon, Korea ]
Corresponding Author